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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 8, Iss. 1 — Jan. 1, 2001
  • pp: 2–10

Confocal microscopy and multi-photon excitation microscopy of human skin in vivo

Barry R. Masters and Peter T. C. So  »View Author Affiliations


Optics Express, Vol. 8, Issue 1, pp. 2-10 (2001)
http://dx.doi.org/10.1364/OE.8.000002


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Abstract

This paper compares tandem scanning reflected light confocal microscopy and multi-photon excitation microscopy for the observation of human skin in vivo. Tandem scanning confocal light microscopy based on a white light source can provide video-rate image acquisition from the skin surface to the epidermal-dermal junction. Multi-photon excitation is induced by a 80 MHz pulse train of femtosecond laser pulses at 780 nm wave length. This nonlinear microscopic technique is inherently suitable for deep tissue fluorescence imaging. The relative merits of these two techniques can be identified by comparing movies of optical sections obtained from the forearm skin of the same volunteer.

© Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.0180) Microscopy : Microscopy
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Research Papers

History
Original Manuscript: October 30, 2000
Published: January 1, 2001

Citation
Barry Masters and Peter So, "Confocal microscopy and multi-photon excitation microscopy of human skin in vivo," Opt. Express 8, 2-10 (2001)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-8-1-2


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References

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