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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 9, Iss. 9 — Oct. 22, 2001
  • pp: 444–453

Large-image-format computed tomography imaging spectrometer for fluorescence microscopy

Bridget K. Ford, Michael R. Descour, and Ronald M. Lynch  »View Author Affiliations


Optics Express, Vol. 9, Issue 9, pp. 444-453 (2001)
http://dx.doi.org/10.1364/OE.9.000444


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Abstract

Multispectral imaging has significantly enhanced the analysis of fixed specimens in pathology and cytogenetics. However, application of this technology to in vivo studies has been limited. This is due in part to the increased temporal resolution required to analyze changes in cellular function. Here we present a non-scanning instrument that simultaneously acquires full spectral information (460 nm to 740 nm) from every pixel within its 2-D field of view (200 µm×200 µm) during a single integration time (typically, 2 seconds). The current spatial and spectral sampling intervals of the spectrometer are 0.985 µm and 5 nm, respectively. These properties allow for the analysis of physiological responses within living biological specimens.

© Optical Society of America

OCIS Codes
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(180.2520) Microscopy : Fluorescence microscopy
(300.6190) Spectroscopy : Spectrometers

ToC Category:
Research Papers

History
Original Manuscript: September 5, 2001
Published: October 22, 2001

Citation
Bridget Ford, Michael Descour, and Ronald Lynch, "Large-image-format computed tomography imaging spectrometer for fluorescence microscopy," Opt. Express 9, 444-453 (2001)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-9-9-444


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