OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 26 — Dec. 24, 2007
  • pp: 17827–17841

Optics InfoBase > Optics Express > Volume 15 > Issue 26 > Depth-resolved optical imaging of transmural electrical propagation in perfused heart

Depth-resolved optical imaging of transmural electrical propagation in perfused heart

Elizabeth M. C. Hillman, Olivier Bernus, Emily Pease, Matthew B. Bouchard, and Arkady Pertsov  »View Author Affiliations


Optics Express, Vol. 15, Issue 26, pp. 17827-17841 (2007)
http://dx.doi.org/10.1364/OE.15.017827


View Full Text Article

Enhanced HTML    Acrobat PDF (3030 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We present a study of the 3-dimensional (3D) propagation of electrical waves in the heart wall using Laminar Optical Tomography (LOT). Optical imaging contrast is provided by a voltage sensitive dye whose fluorescence reports changes in membrane potential. We examined the transmural propagation dynamics of electrical waves in the right ventricle of Langendorf perfused rat hearts, initiated either by endo-cardial or epi-cardial pacing. 3D images were acquired at an effective frame rate of 667Hz. We compare our experimental results to a mathematical model of electrical transmural propagation. We demonstrate that LOT can clearly resolve the direction of propagation of electrical waves within the cardiac wall, and that the dynamics observed agree well with the model of electrical propagation in rat ventricular tissue.

© 2007 Optical Society of America

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 28, 2007
Revised Manuscript: November 14, 2007
Manuscript Accepted: November 18, 2007
Published: December 13, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Elizabeth M. C. Hillman, Olivier Bernus, Emily Pease, Matthew B. Bouchard, and Arkady Pertsov, "Depth-resolved optical imaging of transmural electrical propagation in perfused heart," Opt. Express 15, 17827-17841 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-26-17827


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. R. A. Gray, A. M. Pertsov, and J. Jalife, "Spatial and temporal organization during cardiac fibrillation," Nature 392, 75-78 (1998). [CrossRef] [PubMed]
  2. J. Kalifa, M. Klos, S. Zlochiver, S. Mironov, K. Tanaka, N. Ulahannan, M. Yamazaki, J. Jalife, and O. Berenfeld, "Endoscopic fluorescence mapping of the left atrium: A novel experimental approach for high resolution endocardial mapping in the intact heart," Heart Rhythm. 4, 916-924 (2007). [CrossRef] [PubMed]
  3. I. Tasaki, A. Watanabe, R. Sandlin, and L. Carnay, "Changes in fluorescence, turbidity and birefringence associated with nerve excitation," Proc. Natl. Acad. Sci. 61, 883-888 (1968). [CrossRef] [PubMed]
  4. L. M. Loew, S. Scully, L. Simpson, and A. S. Waggoner, "Evidence for a charge-shift electrochromic mechanism in a probe of membrane potential," Nature. 281, 497-499 (1979). [CrossRef] [PubMed]
  5. E. Fluhler, V. G. Burnham and L. M. Loew, "Spectra, membrane binding and potentiometric responses of new charge shift probes," Biochemistry. 24, 5749-5755 (1985). [CrossRef] [PubMed]
  6. D. Shoham, D. E. Glaser, A. Arieli, T. Kenet, C. Wijnbergen, Y. Toledo, R. Hildesheim, and A. Grinvald, "Imaging cortical dynamics at high spatial and temporal resolution with novel blue voltage-sensitive dyes," Neuron. 24, 791-802 (1999). [CrossRef]
  7. A. Matiukas, B. G. Mitrea, A. M. Pertsov, J. P. Wuskell, M. D. Wei, J. Watras, A. C. Millard, and L. M. Loew, "New near-infrared optical probes of cardiac electrical activity," Am. J. Physiol. Heart Circ. Physiol. 290, H2633-H2643 (2006). [CrossRef] [PubMed]
  8. D. S. Rosenbaum and J. Jalife, Optical mapping of cardiac excitation and arrhythmias, (Armonk, N Y, Futura Publishing Company, Inc. 2001).
  9. I. R. Efimov, V. P. Nikolski, and G. Salama, "Optical imaging of the heart," Circ. Res. 95, 21-33 (2004). [CrossRef] [PubMed]
  10. W. Baxter, S. F. Mironov, A. V. Zaitsev, A. M. Pertsov and J. Jalife, "Visualizing excitation waves in cardiac muscle using transillumination," Biophys. J. 80, 516-530 (2001). [CrossRef] [PubMed]
  11. S. D. Girouard, K. R. Laurita and D. S. Rosenbaum, "Unique properties of cardiac action potentials recorded with voltage-sensitive dyes," J. Cardiovasc. Electrophysiol. 7, 1024-1038 (1996). [CrossRef] [PubMed]
  12. L. Ding, R. Splinter, and S. B. Knisley, "Quantifying spatial localization of optical mapping using Monte Carlo simulations," IEEE Trans. Biomed. Eng. 48, 1098-1107 (2001). [CrossRef] [PubMed]
  13. D. L. Janks and B. J. Roth, "Averaging over depth during optical mapping of unipolar stimulation," IEEE Trans. Biomed. Eng. 49, 1051-1054 (2002). [CrossRef] [PubMed]
  14. T. J. Huppert, R. D. Hoge, A. M. Dale, M. A. Franceschini, and D. A. Boas, "Quantitative spatial comparison of diffuse optical imaging with blood oxygen level-dependent and arterial spin labeling-based functional magnetic resonance imaging," J. Biomed. Opt. 11, 064018 (2006). [CrossRef]
  15. C. J. Hyatt, S. F. Mironov, F. J. Vetter, C. W. Zemlin and A. M. Pertsov, "Optical action potential upstroke morphology reveals near-surface transmural propagation direction," Circ. Res. 97, 277-284 (2005). [CrossRef] [PubMed]
  16. D. Streeter, Handbook of Physiology, (Bethesda, MD, American Physiological Society, 1979).
  17. O. Bernus, M. Wellner, and A. M. Pertsov, "Intramural wave propagation in cardiac tissue: asymptotic solutions and cusp waves," Phys. Rev. E 70, 061913 (2004). [CrossRef]
  18. O. Berenfeld and A. M. Pertsov, "Dynamics of intramural scroll waves in three-dimensional continuous myocardium with rotational anisotropym," J. Theor. Biol. 199, 383-394 (1999). [CrossRef] [PubMed]
  19. O. Bernus, K. S. Mukund, and A. M. Pertsov, "Detection of intramyocardial scroll waves using absorptive transillumination imaging," J. Biomed. Opt. 12, 14035 (2007). [CrossRef]
  20. A. M. Pertsov, "Scroll waves in three-dimensional cardiac muscle," in Cardiac Electrophysiology: from cell to bedside, D. P. Zipes, and J. Jalife, eds., (Philadelphia, PA, Saunders, 2000), pp. 336-344.
  21. E. M. C. Hillman, D. A. Boas, A. M. Dale, and A. K. Dunn, "Laminar Optical Tomography: demonstration of millimeter-scale depth-resolved imaging in turbid media," Opt. Lett. 29, 1650-1652 (2004). [CrossRef] [PubMed]
  22. E. M. C. Hillman, A. Devor, M. Bouchard, A. K. Dunn, G. W. Krauss, J. Skoch, B. J. Bacskai, A. M. Dale, and D. A. Boas, "Depth-resolved Optical Imaging and Microscopy of Vascular Compartment Dynamics during Somatosensory Stimulation," Neuroimage. 35, 89-104 (2007). [CrossRef] [PubMed]
  23. E. M. C. Hillman, A. Devor, A. K. Dunn, and D. A. Boas, "Laminar optical tomography: high-resolution 3D functional imaging of superficial tissues," Proc. SPIE 6143, 61431M (2006). [CrossRef]
  24. A. Nygren, C. Kondo, R. B. Clark, and W. R. Giles, "Voltage-sensitive dye mapping in Langendorff-perfused rat hearts," Am. J. Physiol. Heart Circ. Physiol. 284, H892-H902 (2003).
  25. S. V. Pandit, R. B. Clark, W. R. Giles, and S. S. Demir, "A mathematical model of action potential heterogeneity in adult rat left ventricular myocytes," Biophys. J. 81, 3029-3051 (2001). [CrossRef] [PubMed]
  26. H. D. Himel and S. B. Knisley, "Comparison of optical and electrical mapping of fibrillation," Physiol. Meas. 28, 707-719 (2007). [CrossRef] [PubMed]
  27. A. K. Dunn and D. A. Boas, "Transport-based image reconstruction in turbid media with small source-detector separations," Opt. Lett. 25, 1777-1779 (2000). [CrossRef]
  28. V. Ntziachristos, C.-H. Tung, C. Bremer, and R. Weissleder, "Fluorescence molecular tomography resolves protease activity in vivo," Nat. Med. 8, 757 -761 (2002). [CrossRef] [PubMed]
  29. M. S. Patterson and B. W. Pogue, "Mathematical model for time-resolved and frequency-domain fluorescence spectroscopy in biological tissues," Appl. Opt. 33, 1963-1974 (1994). [CrossRef] [PubMed]
  30. O. Bernus, M. Wellner, S. F. Mironov, and A. M. Pertsov, "Simulation of voltage-sensitive optical signals in three-dimensional slabs of cardiac tissue: application to transillumination and coaxial imaging methods," Phys. Med. Biol. 50, 215-229 (2005). [CrossRef] [PubMed]
  31. C. J. Hyatt, S. F. Mironov, M. Wellner, O. Berenfeld, A. K. Popp, D. A. Weitz, J. Jalife, and A. M. Pertsov, "Synthesis of voltage-sensitive fluorescence signals from three-dimensional myocardial activation patterns," Biophys. J. 85, 2673-2683 (2003). [CrossRef] [PubMed]
  32. J. P. Culver, T. Durduran, D. Furuya, C. Cheung, J. H. Greenberg, and A. G. Yodh, "Diffuse optical tomography of cerebral blood flow, oxygenation, and metabolism in rat during focal ischemia," J. Cereb. Blood Flow Metab. 23, 911-924 (2003). [CrossRef] [PubMed]
  33. J. P. Culver, A. M. Siegel, J. J. Stott, and D. A. Boas, "Volumetric diffuse optical tomography of brain activity," Opt. Lett. 28, 2061-2063 (2003). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Multimedia

Multimedia FilesRecommended Software
» Media 1: AVI (1089 KB)     
» Media 2: AVI (1620 KB)     
» Media 3: AVI (1639 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited