Abstract

A new method to perform simultaneously three dimensional optical sectioning and optical manipulation is presented. The system combines a multi trap optical tweezers with a video microscope to enable axial scanning of living cells while maintaining the trapping configuration at a fixed position. This is achieved compensating the axial movement of the objective by shaping the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. Our method has been validated in three different experimental configurations. In the first, we decouple the position of a trapping plane from the axial movements of the objective and perform optical sectioning of a circle of beads kept on a fixed plane. In a second experiment, we extend the method to living cell microscopy by showing that mechanical constraints can be applied on the dorsal surface of a cell whilst performing its fluorescence optical sectioning. In the third experiment, we trapped beads in a three dimensional geometry and perform, always through the same objective, an axial scan of the volume delimited by the beads.

© 2005 Optical Society of America

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History
Original Manuscript: January 13, 2005
Revised Manuscript: February 17, 2005
Published: March 7, 2005

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Author Affiliations

Valentina Emiliani, Dan Cojoc, Enrico Ferrari, Valeria Garbin, Christiane Durieux, Maite Coppey-Moisan

Institut Jacqus Monod, INFM-TASC

Enzo Di Fabrizio

Institut Jacqus Monod, INFM-TASC and University of Magna Graecia Viale Europa Campus Germaneto cz Italy

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