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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 17 — Aug. 20, 2007
  • pp: 11020–11032

The interaction of lipopolysaccharide with membrane receptors on macrophages pre-treated with extract of Reishi polysaccharides measured by optical tweezers

Ming-Tzo Wei, Kuo-Feng Hua, Jowey Hsu, Artashes Karmenyan, Kai-Yu Tseng, Chi-Huey Wong, Hsien-Yeh Hsu, and Arthur Chiou  »View Author Affiliations

Optics Express, Vol. 15, Issue 17, pp. 11020-11032 (2007)

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Lipopolysaccharide (LPS), one of the cell wall components of Gram-negative bacteria, is recognized by and interacted with receptors on macrophages. In this paper, we report the trapping of LPS-coated polystyrene particles via optical tweezers and measured its interaction with murine macrophages (J774A.1 cells) for cells pre-treated with extract of Reishi polysaccharides (EORP) vs. those without EORP treatment. Our experimental results indicate that the cellular affinity for LPS increases when the macrophage is pretreated with EORP. We demonstrate for the first time by conventional biological methods and by tracking the dynamics of optically-trapped LPS-coated particles interacting with J774A.1 cells, that EORP not only enhances J774A.1 cells surface expression of TLR4 and CD14, two receptors on macrophages, as well as LPS binding and phagocytosis internalization, but also reduces the adhesion time constant and increases the force constant of the binding interaction. The application of optical tweezers allows us to study the effect on a single cell quantitatively in real-time with a spatial resolution ~1µm within a single cell.

© 2007 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 1, 2007
Revised Manuscript: July 29, 2007
Manuscript Accepted: August 14, 2007
Published: August 17, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Ming-Tzo Wei, Kuo-Feng Hua, Jowey Hsu, Artashes Karmenyan, Kai-Yu Tseng, Chi-Huey Wong, Hsien-Yeh Hsu, and Arthur Chiou, "The interaction of lipopolysaccharide with membrane receptors on macrophages pretreated with extract of Reishi polysaccharides measured by optical tweezers," Opt. Express 15, 11020-11032 (2007)

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