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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 23 — Nov. 17, 2003
  • pp: 3074–3079

Collinear optical coherence and confocal fluorescence microscopies for tissue engineering

J. P. Dunkers, M. T. Cicerone, and N. R. Washburn  »View Author Affiliations

Optics Express, Vol. 11, Issue 23, pp. 3074-3079 (2003)

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Tissue engineered medical products (TEMPs) are often three-dimensional (3D) hybrid materials consisting of a porous scaffold upon which the tissue is grown. However, monitoring of the developing tissue deep within the scaffold is hampered by its turbidity. We have sought new ways to probe the interior of the scaffold with the same resolution as conventional laser scanning confocal microscopy but with greater sensitivity. We present a novel application of optical coherence microscopy (OCM) by combining it with confocal fluorescence microscopy (CFM) to gather simultaneous structural and functional information on TEMPs in a registered fashion. In this work, we describe the collinear OCM and CFM instrument. We demonstrate the utility of this dual-mode technique for TEMPs by imaging fluorescently stained osteoblasts cultured in a polymeric TEMP.

© 2003 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0180) Medical optics and biotechnology : Microscopy
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Research Papers

Original Manuscript: October 1, 2003
Revised Manuscript: October 31, 2003
Published: November 17, 2003

J. Dunkers, M. Cicerone, and N. Washburn, "Collinear optical coherence and confocal fluorescence microscopies for tissue engineering," Opt. Express 11, 3074-3079 (2003)

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