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Optics Express

Optics Express

  • Editor: J. H. Eberly
  • Vol. 8, Iss. 1 — Jan. 1, 2001
  • pp: 2–10

Confocal microscopy and multi-photon excitation microscopy of human skin in vivo

Barry R. Masters and Peter T. C. So  »View Author Affiliations

Optics Express, Vol. 8, Issue 1, pp. 2-10 (2001)

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This paper compares tandem scanning reflected light confocal microscopy and multi-photon excitation microscopy for the observation of human skin in vivo. Tandem scanning confocal light microscopy based on a white light source can provide video-rate image acquisition from the skin surface to the epidermal-dermal junction. Multi-photon excitation is induced by a 80 MHz pulse train of femtosecond laser pulses at 780 nm wave length. This nonlinear microscopic technique is inherently suitable for deep tissue fluorescence imaging. The relative merits of these two techniques can be identified by comparing movies of optical sections obtained from the forearm skin of the same volunteer.

© Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.0180) Microscopy : Microscopy
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Research Papers

Original Manuscript: October 30, 2000
Published: January 1, 2001

Barry Masters and Peter So, "Confocal microscopy and multi-photon excitation microscopy of human skin in vivo," Opt. Express 8, 2-10 (2001)

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  1. B. R. Masters, P. T. C. So, and E. Gratton, "Optical biopsy of in vivo human skin: multiphoton excitation microscopy," Lasers Med. Sci. 13,196-203 (1998). [CrossRef]
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  3. B. R. Masters, P. T. C. So, K. H. Kim, C. Buehler, and E. Gratton, "Multiphoton excitation microscopy, confocal microscopy, and spectroscopy of living cells and tissues: functional metabolic imaging of human skin in vivo," in Methods in Enzymology, Confocal Microscopy, vol. 307, P. M. Conn, ed. (Academic Press, New York, 1999) Chap. 29. [CrossRef]
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  27. P. T. C. So, H. Kim, I. E. Kochevar, "Two-photon deep tissue ex vivo imaging of mouse dermal and subcutaneous structures," Opt. Express 3, 339-350 (1998). http://www.opticsexpress.org/oearchive/source/6200.htm [CrossRef] [PubMed]
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