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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 10, Iss. 1 — Jan. 14, 2002
  • pp: 35–40

Longitudinal imaging in biological tissues with a single laser shot correlation system

E. Bordenave, E. Abraham, G. Jonusauskas, J. Oberlé, and C. Rullière  »View Author Affiliations

Optics Express, Vol. 10, Issue 1, pp. 35-40 (2002)

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We demonstrate the potential of a new optical imaging system to directly obtain a longitudinal slice of a biological sample. The system, based on a single-shot optical correlator, operates a time-to-space conversion and an optical time-gating by sum-frequency generation in a nonlinear crystal. Owing to the high speed acquisition of the technique, internal structures of in-vivo tissues can be imaged at video rate. With this apparatus, we recorded longitudinal images of ex vivo mouse ear and in vivo human skin with a depth resolution of approximately 15 μm.

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OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(190.7110) Nonlinear optics : Ultrafast nonlinear optics

ToC Category:
Research Papers

Original Manuscript: December 10, 2001
Published: January 14, 2002

E. Bordenave, Emmanuel Abraham, G. Jonusauskas, J. Oberle, and Claude Rulliere, "Longitudinal imaging in biological tissues with a single laser shot correlation system," Opt. Express 10, 35-40 (2002)

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  1. B.R. Masters and P.T.C. So, "Confocal microscopy and multi-photon excitation microscopy of human skin in vivo," Opt. Express 8, 2-10 (2001), <a href="http://www.opticsexpress.org/oearchive/source/27001.htm">http://www.opticsexpress.org/oearchive/source/27001.htm</a> [CrossRef] [PubMed]
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