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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 10, Iss. 17 — Aug. 26, 2002
  • pp: 893–898

Axial superresolution with ultrahigh aperture lenses

Carlo Mar Blanca and Stefan W. Hell  »View Author Affiliations

Optics Express, Vol. 10, Issue 17, pp. 893-898 (2002)

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We explore the current limits of the axial resolution of optical sectioning microscopy using a single lens, by combining the resolving power of novel 1.45 numerical aperture oil immersion lenses with superresolving binary aperture filters. We quantify the axial resolution brought about by the increase in semiaperture angle to α max =72.8° and demonstrate an absolute gain in axial resolution through binary pupil filters. Implemented in a confocalized two-photon excitation microscope, the combination of both effectively improves the axial resolution to 330 nm full-width-half-maximum, which is by 34% over that of a standard 1.4 NA system.

© 2002 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Research Papers

Original Manuscript: June 4, 2002
Revised Manuscript: August 21, 2002
Published: August 26, 2002

Carlo Blanca and Stefan Hell, "Axial superresolution with ultrahigh aperture lenses," Opt. Express 10, 893-898 (2002)

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