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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 10, Iss. 26 — Dec. 30, 2002
  • pp: 1557–1562

Fluorescence lifetime tomography of turbid media based on an oxygen-sensitive dye

Eric Shives, Yong Xu, and Huabei Jiang  »View Author Affiliations

Optics Express, Vol. 10, Issue 26, pp. 1557-1562 (2002)

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We present for the first time experimental images of fluorescence lifetime distribution using model-based reconstruction. The lifetime distribution in our phantom experiments was realized through using an oxygen-sensitive dye [Sn(IV)Chlorin-e6-Cl2-3Na (SCCN)] whose lifetime varied with the oxygen concentration provided in the target and background media. The fluorescence tomographic data was obtained using our multi-channel frequency-domain system. Spatial maps of fluorescence lifetime were achieved with a finite element based reconstruction algorithm.

© 2002 Optical Society of America

OCIS Codes
(110.6960) Imaging systems : Tomography
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Research Papers

Original Manuscript: September 17, 2002
Revised Manuscript: December 17, 2002
Published: December 30, 2002

Eric Shives, Yong Xu, and Huabei Jiang, "Fluorescence lifetime tomography of turbid media based on an oxygen-sensitive dye," Opt. Express 10, 1557-1562 (2002)

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