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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 10, Iss. 8 — Apr. 22, 2002
  • pp: 370–375

Picosecond time-gated microscopy of UV-damaged plant tissue

S. Rehman and P. B. Lukins  »View Author Affiliations

Optics Express, Vol. 10, Issue 8, pp. 370-375 (2002)

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We demonstrate that picosecond time-gated fluorescence microscopy can be used to monitor subtle changes in the kinetics and spatial distribution of perturbations to the molecular and cellular structure of plant tissue caused by ultraviolet radiation. Single-molecule experiments on Photosystem II and chloroplast preparations give picosecond fluorescence decay kinetics that are similar to those obtained previously on bulk samples. For green plant leaves, localized and well-defined cellular structure is seen for normal material whereas relatively diffuse and non-specific features are seen after UV-irradiation indicating significant UV-induced rupture of the cellular structure. The changes in the chlorophyll fluorescence decay kinetics indicate uncoupling of chlorophyll molecules in the light-harvesting system leading to inhibition of energy reorganization and transfer in the antennae and subsequent exciton transfer to the reaction centers.

© Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.2520) Microscopy : Fluorescence microscopy
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Research Papers

Original Manuscript: December 21, 2001
Revised Manuscript: April 16, 2002
Published: April 22, 2002

S. Rehman and Philip Lukins, "Picosecond time-gated microscopy of UV-damaged plant tissue," Opt. Express 10, 370-375 (2002)

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