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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 10 — May. 19, 2003
  • pp: 1123–1130

Practical implementation of adaptive optics in multiphoton microscopy

P. N. Marsh, D. Burns, and J. M. Girkin  »View Author Affiliations

Optics Express, Vol. 11, Issue 10, pp. 1123-1130 (2003)

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A dedicated two-photon microscope incorporating adaptive-optic correction of specimen-induced aberrations is presented. Wavefront alteration of the scanning laser beam was achieved by use of a micromachined deformable mirror. Post scan head implementation produces a compact module compatible with the Bio-Rad MRC-600 scan head. Automatic aberration correction using feedback from the multiphoton fluorescence intensity allowed the adaptive optic to extend the imaging depth attainable in both artificial and biological refractive-index mismatched samples. With a 1.3-NA, ×40, Nikon oil immersion objective, the imaging depth in water was extended from approximately 3.4 to 46.2 µm with a resolution defined by a FWHM axial point-spread function of 1.25 µm.

© 2003 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(180.0180) Microscopy : Microscopy

ToC Category:
Research Papers

Original Manuscript: March 28, 2003
Revised Manuscript: April 25, 2003
Published: May 19, 2003

P. Marsh, D. Burns, and J. Girkin, "Practical implementation of adaptive optics in multiphoton microscopy," Opt. Express 11, 1123-1130 (2003)

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