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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 12 — Jun. 16, 2003
  • pp: 1385–1391

Multiphoton plasmon-resonance microscopy

Dvir Yelin, Dan Oron, Stephan Thiberge, Elisha Moses, and Yaron Silberberg  »View Author Affiliations


Optics Express, Vol. 11, Issue 12, pp. 1385-1391 (2003)
http://dx.doi.org/10.1364/OE.11.001385


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Abstract

A novel method for detection of noble-metal nanoparticles by their nonlinear optical properties is presented and applied for specific labeling of cellular organelles. When illuminated by laser light in resonance with their plasmon frequency these nanoparticles generate an enhanced multiphoton signal. This enhanced signal is measured to obtain a depth-resolved image in a laser scanning microscope setup. Plasmon-resonance images of both live and fixed cells, showing specific labeling of cellular organelles and membranes, either by two-photon autofluorescence or by third-harmonic generation, are presented.

© 2003 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

ToC Category:
Research Papers

History
Original Manuscript: April 30, 2003
Revised Manuscript: June 1, 2003
Published: June 16, 2003

Citation
Dvir Yelin, Dan Oron, Stephan Thiberge, Elisha Moses, and Yaron Silberberg, "Multiphoton plasmon-resonance microscopy," Opt. Express 11, 1385-1391 (2003)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-11-12-1385


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