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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 12 — Jun. 16, 2003
  • pp: 1385–1391

Multiphoton plasmon-resonance microscopy

Dvir Yelin, Dan Oron, Stephan Thiberge, Elisha Moses, and Yaron Silberberg  »View Author Affiliations

Optics Express, Vol. 11, Issue 12, pp. 1385-1391 (2003)

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A novel method for detection of noble-metal nanoparticles by their nonlinear optical properties is presented and applied for specific labeling of cellular organelles. When illuminated by laser light in resonance with their plasmon frequency these nanoparticles generate an enhanced multiphoton signal. This enhanced signal is measured to obtain a depth-resolved image in a laser scanning microscope setup. Plasmon-resonance images of both live and fixed cells, showing specific labeling of cellular organelles and membranes, either by two-photon autofluorescence or by third-harmonic generation, are presented.

© 2003 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(180.5810) Microscopy : Scanning microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

ToC Category:
Research Papers

Original Manuscript: April 30, 2003
Revised Manuscript: June 1, 2003
Published: June 16, 2003

Dvir Yelin, Dan Oron, Stephan Thiberge, Elisha Moses, and Yaron Silberberg, "Multiphoton plasmon-resonance microscopy," Opt. Express 11, 1385-1391 (2003)

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  1. W. C. W. Chan, S. Nie, ???Quantum dot bioconjugates for ultrasensitive nonisotopic detection,??? Science 281, 2016 (1998). [CrossRef] [PubMed]
  2. M. Bruchez Jr., M. Moronne, P. Gin, S. Weiss, A. P. Alivisatos, ???Semiconductor nanocrystals as fluorescent biological labels,??? Science 281, 2013 (1998). [CrossRef]
  3. S.J. Oldenburg, S.L. Westcott, R.D. Averitt, N.J. Halas, ???Infrared extinction properties of gold nanoshells,??? J. Chem. Phys. 111, 4729 (1999). [CrossRef]
  4. J. Aizpurua, P. Hanarp, D. S. Sutherland, M. Kall, G. W. Bryant, F. J. Garcia de Abajo, ???Optical properties of gold nanorings,??? Phys. Rev. Lett. 90, 057401 (2003). [CrossRef] [PubMed]
  5. D. Boyer, P. Tamarat, A. Maali, B. Lounis, M. Orrit, ???Photothermal imaging of nanometer-sized metal particles among scatterers,??? Science 297, 1160 (2002). [CrossRef] [PubMed]
  6. J.T. Golab, J.R. Sprague, K.T. Carron, G.C. Schatz, R.P. Van Duyne,???A Surface enhanced hyper-Raman scattering study of pyridine adsorbed onto silver: experiment and theory,??? J. Chem. Phys. 888, 7942 (1988). [CrossRef]
  7. H. Kano, S. Kawata, ???Two-photon-excited fluorescence enhanced by a surface plasmon,??? Opt. Lett. 21, 1848 (1996). [CrossRef] [PubMed]
  8. B. Lamprecht, J.R. Krenn, A. Leitner, F.R. Aussenegg, ???Resonant and off-resonant light-driven plasmons in metal nanoparticles studied by femtosecond-resolution third-harmonic generation,??? Phys. Rev. Lett. 83, 4421 (1999). [CrossRef]
  9. G. Peleg, A. Lewis, O. Bouevitch, L. Loew, D. Parnas, M. Linial, ???Gigantic optical non-linearities from nanopartical-enhanced molecular probes with potential for selectively imaging the structure and physiology of nanometric regions in cellular systems,??? Bioimaging 4, 215 (1996). [CrossRef]
  10. W. Denk, J.H. Strickler, W.W. Webb, ???Two-photon laser scanning fluorescence microscopy,??? Science 248, 73 (1990). [CrossRef] [PubMed]
  11. S. Maiti, J.B. Shear, R.M.Williams, W.R. Zipfel, W.W.Webb, ???Measuring serotonin distribution in live cells with three-photon excitation,??? Science 275, 530 (1997). [CrossRef] [PubMed]
  12. D. Yelin, Y. Silberberg, ???Laser scanning third-harmonic-generation microscopy in biology,??? Opt. Express 5, 169 (1999). [CrossRef] [PubMed]
  13. M. Muller, J. Squier, K.R. Wilson, G.J. Brakenhoff,???3D-microscopy of transparent objects using third-harmonic generation,??? J. Microsc. 191, 266 (1998). [CrossRef] [PubMed]
  14. A. Zumbusch, G.R. Holtom, X.S. Xie, ???Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering,??? Phys. Rev. Lett. 82, 4142 (1999). [CrossRef]
  15. S. Nie, S.R. Emory, ???Probing single molecules and single nanoparticles by surface-enhanced Raman scattering,??? Science 275, 1102 (1997). [CrossRef] [PubMed]
  16. G. Peleg, A. Lewis, M. Linial, L.M. Loew, ???Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites,??? Proc. Natl. Acad. Sci. USA 96, 6700 (1999). [CrossRef] [PubMed]
  17. K. Kneipp et al., ???Surface-enhanced Raman spectroscopy in single living cells using gold nanoparticles,??? Appl. Spec. 56, 150 (2002). [CrossRef]
  18. B. J. Messinger, K. Ulrich von Raben, R.K. Chang, P.W. Barber, ???Local fields at the surface of noble metal microspheres,??? Phys. Rev. B 24, 649 (1981). [CrossRef]
  19. E.J. Liang, C. Engert,W. Kiefer, ???Surface-enhanced Raman scattering of pyridine in silver colloids excited in the near-infrared region,??? J. Raman Spec. 24, 775 (1993). [CrossRef]
  20. M. Quinten, ???Local fields close to the surface of nanoparticles and aggregates of nanoparticles,??? Appl. Phys. B 73, 245 (2001). [CrossRef]
  21. D. Yelin, D. Oron, E. Korkotian, M. Segal, Y. Silberberg, ???Third-harmonic microscopy with a Ti:Sapphire laser,??? Appl. Phys. B 74, s97 (2002). [CrossRef]
  22. B. Alberts et al., Molecular Biology of The Cell (Garland Publishing Inc., New York, 1994).
  23. E. Skutelsky, J. Roth, ???Cationic colloidal gold - a new probe for the detection of anionic cell surface sites by electron microscopy,??? J. Histochemistry 34, 693 (1986).
  24. M.S. Bush, G. Allt, ???Blood nerve barrier: distribution of anionic sites on the endothelial plasma membrane and basal lamina,??? Brain Res. 535, 181 (1990). [CrossRef] [PubMed]
  25. Concanavalin A as a Tool, eds. H. Bittiges, H.P. Schnebli, (London, 1976).
  26. R.M. Guasch, C. Guerri, J.-E. O???Connor, ???Study of surface carbohydrates on isolated golgi subfractions by fluorescent-lectin binding and flow cytometry,??? Cytometry 19, 112 (1995); R.M. Guasch, C. Guerri, J.-E. O???Connor, ???Flow cytometric analysis of concanavalin A binding to isolate Golgi fractions from rat liver,??? Exp. Cell Res. 207, 136 (1993). [CrossRef] [PubMed]
  27. I. Virtanen, P. Ekblom, P. Laurila, ???Subcellular compartmentalization of saccharide moieties in cultured normal and malignant cells,??? J. Cell Biol. 85, 429 (1980). [CrossRef] [PubMed]

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