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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 12 — Jun. 16, 2003
  • pp: 1436–1451

Imaging quality assessment of multi-modal miniature microscope

Junwon Lee, Jeremy D. Rogers, Michael R. Descour, Elizabeth Hsu, Jesse S. Aaron, Konstantin Sokolov, and Rebecca R. Richards-Kortum  »View Author Affiliations

Optics Express, Vol. 11, Issue 12, pp. 1436-1451 (2003)

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We are developing a multi-modal miniature microscope (4M device) to image morphology and cytochemistry in vivo and provide better delineation of tumors. The 4M device is designed to be a complete microscope on a chip, including optical, micro-mechanical, and electronic components. It has advantages such as compact size and capability for microscopic-scale imaging. This paper presents an optics-only prototype 4M device, the very first imaging system made of sol-gel material. The micro-optics used in the 4M device has a diameter of 1.3 mm. Metrology of the imaging quality assessment of the prototype device is presented. We describe causes of imaging performance degradation in order to improve the fabrication process. We built a multi-modal imaging test-bed to measure first-order properties and to assess the imaging quality of the 4M device. The 4M prototype has a field of view of 290 µm in diameter, a magnification of -3.9, a working distance of 250 µm and a depth of field of 29.6±6 µm. We report the modulation transfer function (MTF) of the 4M device as a quantitative metric of imaging quality. Based on the MTF data, we calculated a Strehl ratio of 0.59. In order to investigate the cause of imaging quality degradation, the surface characterization of lenses in 4M devices is measured and reported. We also imaged both polystyrene microspheres similar in size to epithelial cell nuclei and cervical cancer cells. Imaging results indicate that the 4M prototype can resolve cellular detail necessary for detection of precancer.

© 2003 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.3000) Imaging systems : Image quality assessment
(350.3950) Other areas of optics : Micro-optics

ToC Category:
Research Papers

Original Manuscript: April 17, 2003
Revised Manuscript: June 6, 2003
Published: June 16, 2003

Junwon Lee, Jeremy Rogers, Michael Descour, Elizabeth Hsu, Jesse Aaron, Konstantin Sokolov, and Rebecca Richards-Kortum, "Imaging quality assessment of multi-modal miniature microscope," Opt. Express 11, 1436-1451 (2003)

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