OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 14 — Jul. 14, 2003
  • pp: 1650–1658

High speed, wide velocity dynamic range Doppler optical coherence tomography (Part II): Imaging in vivo cardiac dynamics of Xenopus laevis

Victor X.D. Yang, Maggie L. Gordon, Emily Seng-Yue, Stewart Lo, Bing Qi, Julius Pekar, Alvin Mok, Brian C. Wilson, and I. Alex Vitkin  »View Author Affiliations


Optics Express, Vol. 11, Issue 14, pp. 1650-1658 (2003)
http://dx.doi.org/10.1364/OE.11.001650


View Full Text Article

Enhanced HTML    Acrobat PDF (1975 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We previously reported a Doppler optical coherence tomography (DOCT) system design [1] for high-speed imaging with wide velocity dynamic range (up to 28.5 dB when acquiring 8 frames per second), operating at 1.3 µm with a coherence length of 13.5 µm. Using a developmental biology model (Xenopus laevis), here we test the DOCT system’s ability to image cardiac dynamics in an embryo in vivo, with a simple hand-held scanner at 4 ~ 16 frames per second. In particular, we show that high fidelity DOCT movies can be obtained by increasing the reference arm scanning rate (~8 kHz). Utilizing a combination of four display modes (B-mode, color-Doppler, velocity variance, and Doppler spectrum), we show that DOCT can detect changes in velocity distribution during heart cycles, measure the velocity gradient in the embryo, and distinguish blood flow Doppler signal from heart wall motions.

© 2003 Optical Society of America

OCIS Codes
(100.6950) Image processing : Tomographic image processing
(110.4500) Imaging systems : Optical coherence tomography
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Research Papers

History
Original Manuscript: May 29, 2003
Revised Manuscript: June 30, 2003
Published: July 14, 2003

Citation
Victor X.D. Yang, Maggie Gordon, Emily Seng-Yue, Stewart Lo, Bing Qi, Julius Pekar, Alvin Mok, Brian Wilson, and I. Vitkin, "High speed, wide velocity dynamic range Doppler optical coherence tomography (Part II): Imaging in vivo cardiac dynamics of Xenopus laevis," Opt. Express 11, 1650-1658 (2003)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-11-14-1650


Sort:  Journal  |  Reset  

References

  1. V. X. Yang, M. L. Gordon, B. Qi, J. Pekar, S. Lo, E. Seng-Yue, A. Mok, B. C. Wilson, and I. A. Vitkin, "High speed, wide velocity dynamic range Doppler optical coherence tomography (Part I): System design, signal processing, and performance," Opt. Express 11, 794-809 (2003), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-7-794.">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-7-794</a>. [CrossRef] [PubMed]
  2. D.Huang, E.A.Swanson, C.P.Lin, J.S.Schuman,W.G.Stinson, W.Chang, M.R.Hee, T.Flotte, K. Gregory, C.A.Puliafito, and J.G.Fujimoto, �??Optical coherence tomography,�?? Science 254, 1178-81 (1991). [CrossRef] [PubMed]
  3. S. A. Boppart, G. J. Tearney, B. E. Bouma, J. F. Southern, M. E. Brenzinski, and J. G. Fujimoto, �??Noninvasive assessment of the developing Xenopus cardiovascular system using optical coherence tomography,�?? Proc. Natl. Acad. Sci. 94, 4256-61 (1997). [CrossRef] [PubMed]
  4. S. Yazdanfar, M.D. Kulkarni, and J.A. Izatt, �??High resolution imaging of in vivo cardiac dynamics using color Doppler optical coherence tomography,�?? Opt. Express 1, 424-31 (1997), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-1-13-424.">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-1-13-424</a>. [CrossRef] [PubMed]
  5. G.J. Tearney, B.E. Bouma, and J.G. Fujimoto, �??High-speed phase- and group-delay scanning with a grating-based phase control delay line,�?? Opt. Lett. 22(23), 1811-3 (1997). [CrossRef]
  6. A.M. Rollins, M.D. Kulkarni, S. Yazdanfar, R. Ung-arunyawee, and J.A. Izatt, �??In vivo video rate optical coherence tomography,�?? Opt. Express 3, 220-29 (1998) <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-6-219.">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-6-219</a>. [CrossRef]
  7. V.Westphal, S.Yazdanfar, A.M.Rollins, and J.A.Izatt, �??Real-time, high velocity-resolution color Doppler optical coherence tomography�??, Opt. Lett. 27(1), 34-6 (2002). [CrossRef]
  8. V.X.D.Yang, M.L.Gordon, A.Mok, Y.Zhao, Z.Chen, R.S.C.Cobbold, B.C.Wilson, and I.A.Vitkin, �??Improved phase-resolved optical Doppler tomography using the Kasai velocity estimator and histogram segmentation,�?? Opt. Comm. 208, 209-214 (2002). [CrossRef]
  9. G. J. Tearney, M. E. Brezinski, B. E. Bouma, S. A. Boppart, C. Pitris, J. F. Southern, and J. G. Fujimoto, �??In vivo endoscopic optical biopsy with optical coherence tomography,�?? Science 276, 2037-9 (1997). [CrossRef] [PubMed]
  10. P. D. Nieuwkoop, and J. Faber, Normal Table of Xenopus laevis (Daudin), Garland, New York (1994).
  11. J.A.Jensen, Estimation of blood velocities using ultrasound (Cambridge, 1996).
  12. S. J. Kolker, U. Tajchman, and D. L. Weeks, �??Confocal imaging of early heart development in Xenopus laevis,�?? Develop. Biol. 218, 64-73 (2000). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Multimedia

Multimedia FilesRecommended Software
» Media 1: MOV (213 KB)     
» Media 2: MOV (481 KB)     
» Media 3: MOV (231 KB)     
» Media 4: MOV (473 KB)     
» Media 5: MOV (1540 KB)     
» Media 6: MOV (914 KB)     
» Media 7: MOV (246 KB)     
» Media 8: MOV (304 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited