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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 14 — Jul. 14, 2003
  • pp: 1695–1701

Selective two-photon microscopy with shaped femtosecond pulses

Igor Pastirk, Johanna M. Dela Cruz, Katherine A. Walowicz, Vadim V. Lozovoy, and Marcos Dantus  »View Author Affiliations

Optics Express, Vol. 11, Issue 14, pp. 1695-1701 (2003)

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Selective two-photon excitation of fluorescent probe molecules using phase-only modulated ultrashort 15-fs laser pulses is demonstrated. The spectral phase required to achieve the maximum contrast in the excitation of different probe molecules or identical probe molecules in different micro-chemical environments is designed according to the principles of multiphoton intrapulse interference (MII). The MII method modulates the probabilities with which specific spectral components in the excitation pulse contribute to the two-photon absorption process due to the dependence of the absorption on the power spectrum of E2(t) [13]. Images obtained from a number of samples using the multiphoton microscope are presented.

© 2003 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(320.0320) Ultrafast optics : Ultrafast optics
(320.5540) Ultrafast optics : Pulse shaping

ToC Category:
Research Papers

Original Manuscript: May 21, 2003
Revised Manuscript: July 3, 2003
Published: July 14, 2003

Igor Pastirk, Johanna Dela Cruz, Katherine Walowicz, Vadim Lozovoy, and Marcos Dantus, "Selective two-photon microscopy with shaped femtosecond pulses," Opt. Express 11, 1695-1701 (2003)

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