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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 15 — Jul. 28, 2003
  • pp: 1740–1745

Axial gain resolution in optical sectioning fluorescence microscopy by shaded-ring filters

M. Martínez-Corral, C. Ibáñez-López, G. Saavedra, and M. T. Caballero  »View Author Affiliations

Optics Express, Vol. 11, Issue 15, pp. 1740-1745 (2003)

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We present a new family of pupil masks to control the axial component of the intensity distribution in the focal region of tightly focused light fields. The filters, which consist of a circular clear pupil with a single shaded ring, allow to control the width of the central lobe of the axial spot together with the residual sidelobes energy. The filters can be applied to improve the optical sectioning capacity of different scanning microscopes.

© 2003 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Research Papers

Original Manuscript: June 19, 2003
Revised Manuscript: July 15, 2003
Published: July 28, 2003

M. Martinez-Corral, C. Ibáñez-López, G. Saavedra, and M. Caballero, "Axial gain resolution in optical sectioning fluorescence microscopy by shaded-ring filters," Opt. Express 11, 1740-1745 (2003)

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