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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 15 — Jul. 28, 2003
  • pp: 1740–1745

Axial gain resolution in optical sectioning fluorescence microscopy by shaded-ring filters

M. Martínez-Corral, C. Ibáñez-López, G. Saavedra, and M. T. Caballero  »View Author Affiliations


Optics Express, Vol. 11, Issue 15, pp. 1740-1745 (2003)
http://dx.doi.org/10.1364/OE.11.001740


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Abstract

We present a new family of pupil masks to control the axial component of the intensity distribution in the focal region of tightly focused light fields. The filters, which consist of a circular clear pupil with a single shaded ring, allow to control the width of the central lobe of the axial spot together with the residual sidelobes energy. The filters can be applied to improve the optical sectioning capacity of different scanning microscopes.

© 2003 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Research Papers

History
Original Manuscript: June 19, 2003
Revised Manuscript: July 15, 2003
Published: July 28, 2003

Citation
M. Martinez-Corral, C. Ibáñez-López, G. Saavedra, and M. Caballero, "Axial gain resolution in optical sectioning fluorescence microscopy by shaded-ring filters," Opt. Express 11, 1740-1745 (2003)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-11-15-1740


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References

  1. J. Pawley (ed.), Handbook of Biological Confocal Microscopy (Plenun, New York, 1995). [CrossRef]
  2. A. Diaspro (ed.) Confocal and Two-Photon Microscopy. Foundations, Applications and Advances (Wiley, New York, 2001).
  3. C. M. Blanca, J. Bewersdorf and S. W. Hell, �??Single sharp spot in fluorescence microscopy of two opposing lenses,�?? Appl. Phys. Lett. 79, 2321-2323 (2001) [CrossRef]
  4. T. A. Klar, E. Engel and S. W. Hell, "Breaking Abbe's diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes", Phys. Rev. E 64: 066613, 1-9 (2001). [CrossRef]
  5. M. A. A. Neil, R. Juskaitis, T. Wilson, Z. J. Laczik and V. Sarafis, "Optimized pupil-plane filters for confocal microscope point-spread function engineering," Opt. Lett. 25, 245-247 (2000). [CrossRef]
  6. Z. Ding, G. Wang, M. Gu, Z. Wang and Z. Fan, "Superresolution with an apodization film in a confocal setup," Appl. Opt. 36, 360-363 (1997). [CrossRef] [PubMed]
  7. C. J. R. Sheppard, "Binary optics and confocal imaging," Opt. Lett. 24, 505-506 (1999). [CrossRef]
  8. M. Martínez-Corral, M. T. Caballero, E. Stelzer and J. Swoger, "Tailoring the axial shape of the PSF using the Toraldo concept," Opt. Express 10, 98-103 (2002). <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-1-98">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-1-98</a> [CrossRef] [PubMed]
  9. G. Boyer, "New class of axially apodizing filters for confocal scanning microscopy," J. Opt. Soc. Am. A 19, 584-589 (2002). [CrossRef]
  10. G. Boyer and V. Sarafis, "Two pinhole superresolution using spatial filters," Optik 112, 177-179 (2001). [CrossRef]
  11. C. M. Blanca and S. W. Hell, �??Axial superresolution with ultrahigh aperture lenses,�?? Opt. Express 10, 893-898 (2002). <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-17-893">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-17-893</a> [CrossRef] [PubMed]
  12. B. Richards and E. Wolf, "Electromagnetic diffraction in optical systems. II. Structure of the image field in an aplanatic system," Proc. Roy. Soc. (London) A 253, 358-379 (1959). [CrossRef]
  13. P. D. Higdon, P. Török and T. Wilson, �??Imaging properties of high aperture multiphoton fluorescence scanning optical microscopes,�?? J. Microsc. 193, 127-141 (1998). [CrossRef]
  14. C. J. R. Sheppard, �??High apertured beams,�?? J. Opt. Soc. Am. A 18, 1579-1587 (2001). [CrossRef]
  15. K. Bahlman and S. W. Hell, �??Electric field depolarization in high aperture focusing with emphasis on annular apertures,�?? J. Microsc. 200, 59-67 (2000). [CrossRef]
  16. M. A. A. Neil, F. Massoumian, R. Juskaitis and T. Wilson, �??A method for the generation of arbitrary complex vector wavefronts,�?? Opt. Lett. 27, 1929-1931 (2002). [CrossRef]
  17. P. Török and F. J. Kao (eds.) Optical Imaging and Microscopy: Techniques and Advanced Systems, (Springer, Heidelberg, 2003).
  18. J. W. M. Chon, X. Gan and M. Gu, �??Splitting of the focal spot of a high-numerical objective in free space,�?? Appl. Phys. Lett. 81, 1576-1579 (2002). [CrossRef]
  19. I. Akduman, U. Brand, J. Grochmalicki, G. Hester, R. Pike and M. Bertero, �??Superresolving masks for incoherent high-NA scanning microscopy in three dimensions,�?? J. Opt. Soc. Am. A 15, 2275-2287 (1998). [CrossRef]
  20. D. M. de Juana, J. E. Oti, V. F. Canales and M. P. Cagigal, �??Design of superresolving continuous phase filters,�?? Opt. Lett. 28, 607-609 (2003). [CrossRef] [PubMed]
  21. S. Mezouari and A. R. Harvey, �??Phase pupil functions for reduction of defocus and spherical aberration,�?? Opt. Lett. 28, 771-773 (2003). [CrossRef] [PubMed]
  22. H. Liu, Y. Yan, D. Yi and G. Jin, �??Design of three-dimensional superresolution filters and limits of axial optical superresolution,�?? Appl. Opt. 42, 1463-1476 (2003). [CrossRef] [PubMed]
  23. G. Toraldo di Francia, "Nuovo pupille superresolventi," Atti Fond. Giorgio Ronchi 7, 366-372 (1952).
  24. J. Campos, J. C. Escalera, C. J. R. Sheppard and M. J. Yzuel, �??Axially invariant pupil filters,�?? J. Mod. Opt. 47, 57-68 (2000).
  25. S. Grill and E. H. K. Stelzer, "Method to calculate lateral and axial gain factors of optical setups with a large solid angle," J. Opt. Soc. Am. A 16, 2658-2665 (1999). [CrossRef]
  26. C. J. R. Sheppard, "Leaky annular pupils for improved axial imaging," Optik 99, 32-34 (1995).
  27. C. J. R. Sheppard and P. Török, �??An electromagnetic theory of imaging in fluorescence microscopy, and imaging in polarization fluorescence microscopy,�?? Bioimaging 5, 205-218 (1997). [CrossRef]
  28. D. Ganic, J. W. M. Chon and M. Gu, �??Effect of numerical aperture on the splitting feature near phase singularities of focused waves,�?? Appl. Phys. Lett. 82, 1527-1528 (2003). [CrossRef]
  29. M. Martínez-Corral, L. Muñoz-Escrivá, M. Kowalczyk and T. Cichocki, "One-dimensional iterative algorithm for three-dimensional point-spread function engineering," Opt. Lett. 26, 1861-1863 (2001). [CrossRef]

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