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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 21 — Oct. 20, 2003
  • pp: 2753–2761

Three-dimensional imaging of the human retina by high-speed optical coherence tomography

Christoph K. Hitzenberger, Peter Trost, Pak-Wai Lo, and Qienyuan Zhou  »View Author Affiliations

Optics Express, Vol. 11, Issue 21, pp. 2753-2761 (2003)

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Conventional optical coherence tomography is based on A-scans, i.e., the fast scan direction is the z-direction. While this technique has been successfully demonstrated for two-dimensional cross sectional imaging of various tissues, it is rather slow if three-dimensional information is to be obtained. We report on a new technique that combines the transverse scanning approach of a confocal scanning laser ophthalmoscope with the depth sectioning capability of OCT. A stable high-frequency carrier is generated by use of an acousto optic modulator, and high frame rate is obtained by using a resonant scanning mirror for the priority scan (x-direction). Our prototype instrument records 64 transverse images consisting of 256×128 pixels in 1.2 seconds, thus providing the fastest retinal 3D OCT scanning system reported so far. We demonstrate the capabilities of our system by measuring and imaging the fovea and the optic nerve head region of healthy human volunteers in vivo.

© 2003 Optical Society of America

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.4470) Medical optics and biotechnology : Ophthalmology

ToC Category:
Research Papers

Original Manuscript: September 15, 2003
Revised Manuscript: October 14, 2003
Published: October 20, 2003

Christoph Hitzenberger, Peter Trost, Pak-Wai Lo, and Qienyuan Zhou, "Three-dimensional imaging of the human retina by high-speed optical coherence tomography," Opt. Express 11, 2753-2761 (2003)

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  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991) [CrossRef] [PubMed]
  2. B. E. Bouma and G. J. Tearney, Handbook of optical coherence tomography (Marcel Dekker, New York, 2002).
  3. A. F. Fercher and C. K. Hitzenberger, "Optical Coherence Tomography," Progr. Opt. 44, 215-302 (2002). [CrossRef]
  4. C. A. Puliafito, M. R. Hee, C. P. Lin, E. Reichel, J. S. Schuman, J. S. Duker, J. A. Izatt, E. A. Swanson, J.G. Fujimoto, "Imaging of macular diseases with optical coherence tomography," Ophthalmology 102, 217-229 (1995). [PubMed]
  5. W. Drexler, O. Findl, R. Menapace, A. Kruger, A. Wedrich, G. Rainer, A. Baumgartner, C. K. Hitzenberger, A. F. Fercher, "Dual beam optical coherence tomography: Signal identification for ophthalmologic diagnosis," J. Biomed. Opt. 3, 55-65 (1998). [CrossRef] [PubMed]
  6. R. Lattanzio, R. Brancato, R. Pierro, F. Bandello, B. Iaccher, T. Fiore, G. Maestranzi, "Macular thickness measured by optical coherence tomography (OCT) in diabetic patients," Eur. J. Ophthalmol. 12, 482-487 (2002).
  7. A. M. Rollins, M. D. Kulkarni, S. Yazdanfar, R. Ung-arunyawee, J. A. Izatt, "In vivo video rate optical coherence tomography," Opt. Express 3, 219-229 (1998), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-6-219">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-6-219</a>. [CrossRef] [PubMed]
  8. A. G. Podoleanu, G. M. Dobre, D. A. Jackson, "En-face coherence imaging using galvanometer scanner modulation," Opt. Lett. 23, 147-149 (1998). [CrossRef]
  9. A. G. Podoleanu, M. Seeger, G. M. Dobre, D. J. Webb, D. A. Jackson, F. Fitzke, "Transversal and longitudinal images from the retina of the living eye using low coherence reflectometry," J. Biomed. Opt. 3, 12-20 (1998) [CrossRef] [PubMed]
  10. H.-W. Wang, A. M. Rollins, J. A. Izatt, "High speed, full field optical coherence microscopy," in Coherence domain optical methods in biomedical science and clinical applications III, V. V. Tuchin and J. A. Izatt, eds., Proc. SPIE 3598, 204-212 (1999). [CrossRef]
  11. R. H. Webb, G. W. Hughes, F. C. Delori, "Confocal scanning laser ophthalmoscope," Appl. Opt. 26, 1492-1499 (1987) [CrossRef] [PubMed]
  12. E. A. Swanson, D. Huang, M. R. Hee, J. G. Fujimoto, C. P. Lin, C. A. Puliafito, "High-speed optical coherence domain reflectometry," Opt Lett 17, 151-153 (1992) [CrossRef] [PubMed]
  13. American national standard for safe use of lasers. ANSI Z 136.1 (Laser Institute of America, Orlando, 2000).
  14. F. I. Feldchtein, G. V. Gelikonov, V. M. Gelikonov, R. R. Iksanov, R. V. Kuranov, A. M. Sergeev, N. D. Gladkova, M. N. Ourutina, J. A. Warren Jr., D. H. Reitze, "In vivo OCT imaging of hard and soft tissue of the oral cavity," Opt. Express 3, 239-250 (1998), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-6-239">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-6-239<a/>. [CrossRef] [PubMed]
  15. S. A. Boppart, B. E. Bouma, C. Pitris, G. J. Tearney, J. F. Southern, M. E. Brezinski, J. G. Fujimoto, "Intraoperative assessment of microsurgery with three-dimensional optical coherence tomography," Radiology 208, 81-86 (1998). [PubMed]
  16. A.G. Podoleanu, J. A. Rogers, D. A. Jackson, S. Dunne, "Three dimensional OCT images from retina and skin," Opt. Express 7, 292-298 (2000), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-7-9-292">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-7-9-292</a>. [CrossRef] [PubMed]
  17. B. M. Hoeling, A. D. Fernandez, R. C. Haskell, E. Huang, W. R. Myers, D. C. Petersen, S. E. Ungersma, R. Wang, M. E. Williams, "An optical coherence microscope for 3-dimensional imaging in developmental biology," Opt. Express 6, 136-146 (2000), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-6-7-136">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-6-7-136</a>. [CrossRef] [PubMed]
  18. P. Massin, G. Duguid, A. Erginay, B. Haouchine, A. Gaudric, "Optical coherence tomography for evaluating diabetic macular edema before and after vitrectomy," Am. J. Ophthalmol. 135, 169-177 (2003) [CrossRef] [PubMed]
  19. C. Strøm, B. Sander, N. Larsen, M. Larsen, H. Lund-Andersen, "Diabetic macular edema assessed with optical coherence tomography and stereo fundus photography," Invest. Ophthalmol. Vis. Sci. 43, 241-245 (2002). [PubMed]

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