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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 22 — Nov. 3, 2003
  • pp: 2964–2969

Point spread function of optical microscopes imaging through stratified media

O. Haeberlé, M. Ammar, H. Furukawa, K. Tenjimbayashi, and P. Török  »View Author Affiliations


Optics Express, Vol. 11, Issue 22, pp. 2964-2969 (2003)
http://dx.doi.org/10.1364/OE.11.002964


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Abstract

We propose a model for imaging point objects through a dielectric interface or stratified media. The model is applicable to conventional and confocal fluorescence microscopy, with single- or multiphoton excitation. An analytical solution is obtained in the form of readily computable functions. When large mismatches occur in the refractive indices of the media of the objective lens and specimen the illumination and detection point spread functions differ significantly, showing that currently used imaging models may fail to correctly predict imaging properties of optical microscopes.

© 2003 Optical Society of America

OCIS Codes
(050.1960) Diffraction and gratings : Diffraction theory
(180.0180) Microscopy : Microscopy
(260.0260) Physical optics : Physical optics

ToC Category:
Research Papers

History
Original Manuscript: September 16, 2003
Revised Manuscript: October 27, 2003
Published: November 3, 2003

Citation
O. Haeberlé, M. Ammar, H. Furukawa, K. Tenjimbayashi, and P. Török, "The point spread function of optical microscopes imaging through stratified media," Opt. Express 11, 2964-2969 (2003)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-11-22-2964


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