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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 23 — Nov. 17, 2003
  • pp: 3093–3099

In vivo developmental biology study using noninvasive multi-harmonic generation microscopy

Shi-Wei Chu, Szu-Yu Chen, Tsung-Han Tsai, Tzu-Ming Liu, Cheng-Yung Lin, Huai-Jen Tsai, and Chi-Kuang Sun  »View Author Affiliations

Optics Express, Vol. 11, Issue 23, pp. 3093-3099 (2003)

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Morphological changes and complex developmental processes inside vertebrate embryos are difficult to observe noninvasively with millimeter-penetration and sub-micrometer-resolution at the same time. By using higher harmonic generation, including second and third harmonics, as the microscopic contrast mechanism, optical noninvasiveness can be achieved due to the virtual-level-transition characteristic. The intrinsic nonlinearity of harmonic generations provides optical sectioning capability while the selected 1230-nm near-infrared light source provides the deep-enetration ability. The complicated development within a ~1.5-mm thick zebrafish (Danio rerio) embryo from initial cell proliferation, gastrulation, to tissue formation can all be observed clearly in vivo without any treatment on the live specimen.

© 2003 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics

ToC Category:
Research Papers

Original Manuscript: August 27, 2003
Revised Manuscript: November 4, 2003
Published: November 17, 2003

Shi-Wei Chu, Szu-Yu Chen, Tsung-Han Tsai, Tzu-Ming Liu, Cheng-Yung Lin, Huai-Jen Tsai, and Chi-Kuang Sun, "In vivo developmental biology study using noninvasive multi-harmonic generation microscopy," Opt. Express 11, 3093-3099 (2003)

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