OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 23 — Nov. 17, 2003
  • pp: 3093–3099

In vivo developmental biology study using noninvasive multi-harmonic generation microscopy

Shi-Wei Chu, Szu-Yu Chen, Tsung-Han Tsai, Tzu-Ming Liu, Cheng-Yung Lin, Huai-Jen Tsai, and Chi-Kuang Sun  »View Author Affiliations


Optics Express, Vol. 11, Issue 23, pp. 3093-3099 (2003)
http://dx.doi.org/10.1364/OE.11.003093


View Full Text Article

Enhanced HTML    Acrobat PDF (1065 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Morphological changes and complex developmental processes inside vertebrate embryos are difficult to observe noninvasively with millimeter-penetration and sub-micrometer-resolution at the same time. By using higher harmonic generation, including second and third harmonics, as the microscopic contrast mechanism, optical noninvasiveness can be achieved due to the virtual-level-transition characteristic. The intrinsic nonlinearity of harmonic generations provides optical sectioning capability while the selected 1230-nm near-infrared light source provides the deep-enetration ability. The complicated development within a ~1.5-mm thick zebrafish (Danio rerio) embryo from initial cell proliferation, gastrulation, to tissue formation can all be observed clearly in vivo without any treatment on the live specimen.

© 2003 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics

ToC Category:
Research Papers

History
Original Manuscript: August 27, 2003
Revised Manuscript: November 4, 2003
Published: November 17, 2003

Citation
Shi-Wei Chu, Szu-Yu Chen, Tsung-Han Tsai, Tzu-Ming Liu, Cheng-Yung Lin, Huai-Jen Tsai, and Chi-Kuang Sun, "In vivo developmental biology study using noninvasive multi-harmonic generation microscopy," Opt. Express 11, 3093-3099 (2003)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-11-23-3093


Sort:  Journal  |  Reset  

References

  1. G. Peleg, A. Lewis, M. Linial, and L. M. Loew, �??Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites,�?? Proc. Natl. Acad. Sci. 96, 6700-6704 (1999). [CrossRef] [PubMed]
  2. S.-W. Chu, I-S. Chen, T.-M. Liu, C.-K. Sun, S.-P. Lee, B.-L. Lin, P.-C. Cheng, M.-X. Kuo, D.-J. Lin, and H.-L. Liu, �??Nonlinear bio-photonic crystal effects revealed with multi-modal nonlinear microscopy,�?? J. Microscopy 208, 190-200 (2002). [CrossRef]
  3. P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, �??Three dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues,�?? Biophys. J. 82, 493-508 (2002). [CrossRef]
  4. M. Müller, J. Squier, K. R. Wilson, and G. J. Brakenhoff, �??3D microscopy of transparent objects using third-harmonic generation,�?? J. Microsc. 191, 266-274 (1998). [CrossRef] [PubMed]
  5. D. Yelin and Y. Silberberg, �??Laser scanning third-harmonic-generation microscopy in biology,�?? Opt. Express 5, 169-175 (1999) <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-5-8-169">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-5-8-169</a> [CrossRef] [PubMed]
  6. L. Canioni, S. Rivet, L. Sarger, R. Barille, P. Vacher, and P. Voisin, �??Imaging of Ca2+ intracellular dynamics with a third-harmonic generation microscope,�?? Opt. Lett. 26, 515-517 (2001). [CrossRef]
  7. A. Y. Louie, M. M. Hüber, E. T. Ahrens, U. Rothbächer, R. Moats, R. E. Jacobs, S. E. Fraser, and T. J. Meade, �??In vivo visualization of gene expression using magnetic resonance imaging,�?? Nat. Biotech. 18, 321-325 (2000). [CrossRef]
  8. F. S. Foster, C. J. Pavlin, K. A. Harasiewicz, D.A. Christopher, and D. H. Turnbull, �??Advances in ultrasound biomicroscopy,�?? Ultrasound in Med. Biol. 26, 1�??27 (2000). [CrossRef]
  9. S. A. Boppart, G. J. Tearney, B. E. Bouma, J. F. Southern, M. E. Brezinski, and J. G. Fujimoto, �??Noninvasive assessment of the developing Xenopus cardiovascular system using optical coherence tomography,�?? Proc. Natl. Acad. Sci. 94, 4256�??4261 (1997). [CrossRef] [PubMed]
  10. T. M. Yelbuz, M. A. Choma, L. Thrane, M. L. Kirby, and J. A. Izatt, �??Optical coherence tomography: A new high-resolution imaging technology to study cardiac development in chick embryos,�?? Circulation 106, 2771-2774 (2002). [CrossRef] [PubMed]
  11. C. Palmes-Saloma and C. Saloma, �??Long-depth imaging of specific gene expressions in whole-mount mouse embryos with single-photon excitation confocal fluorescence microscopy and FISH,�?? J. Struct. Bio. 131, 56�??66 (2000). [CrossRef]
  12. J. M. Squirrell, D. L. Wokosin, J. G. White, and B. D. Bavister, �??Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability,�?? Nat. Biotech. 17, 763-767 (1999). [CrossRef]
  13. C. L. Phillips, L. J. Arend, A. J. Filson, D. J. Kojetin, J. L. Clendenon, S. Fang, and K. W. Dunn, �??Three dimensional imaging of embryonic mouse kidney by two-photon microscopy,�?? Am. J. Pathol. 158, 49-55 (2001). [CrossRef] [PubMed]
  14. R. R. Anderson and J. A. Parish, �??The optics of human skin,�?? J. Invest. Dermat. 77, 13-19 (1981). [CrossRef]
  15. B. E. Bouma, G. J. Tearney, I. P. Bilinsky, B. Golubovic, and J. G. Fujimoto, �??Self-phase-modulated Kerrlens mode-locked Cr:forsterite laser source for optical coherence tomography,�?? Opt. Lett. 21, 1839 (1996). [CrossRef] [PubMed]
  16. S.-W. Chu, I-H. Chen, T.-M. Liu, P. C. Cheng, C.-K. Sun, and B.-L. Lin, �??Multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser,�?? Opt. Lett. 26, 1909-1911 (2001). [CrossRef]
  17. T.-M. Liu, S.-W. Chu, C.-K. Sun, B.-L. Lin, P. C. Cheng, and I. Johnson, �??Multi-photon scanning microscopy using a femtosecond Cr:forsterite laser,�?? Scanning 23, 249-254 (2001). [CrossRef] [PubMed]
  18. A. Seas, V. Petri�?evi�?, and R.R. Alfano, �??Generation of sub-100-fs pulses from a CW mode-locked chromium-doped forsterite laser,�?? Opt. Lett. 17, 937-939 (1992). [CrossRef] [PubMed]
  19. C. B. Kimmel, W. W. Ballard, S. R. Kimmel, B. Ullmann, and T. F. Schilling, �??Stages of embryonic development of the zebrafish,�?? Dev. Dynam. 203, 253-310 (1995). [CrossRef]
  20. J. M. Schins, T. Schrama, J. Squier, G. J. Brakenhoff, and M. Müller, �??Determination of material properties by use of third-harmonic generation microscopy,�?? J. Opt. Soc. Am. B 19, 1627-1634 (2002). [CrossRef]
  21. K. König, P.T.C. So, W.W. Mantulin, and E. Gratton, �??Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes,�?? Opt. Lett. 22, 135-136 (1997). [CrossRef] [PubMed]
  22. A. Schönle and S. W. Hell, �??Heating by absorption in the focus of an objective lens,�?? Opt. Lett. 23, 325-327 (1998) [CrossRef]
  23. I-H. Chen, S.-W. Chu, C.-K. Sun, P. C. Cheng, and B.-L. Lin, �??Wavelength dependent damage in biological multi-photon confocal microscopy: a micro-spectroscopic comparison between femtosecond Ti:sapphire and Cr:forsterite laser sources,�?? Opt. Quantum. Electron 34, 1251-1266 (2002). [CrossRef]
  24. S.-W. Chu, T.-M. Liu, C.-K. Sun, C.-Y. Lin, and H.-J. Tsai, �??Real-time second-harmonic-generation microscopy based on a 2-GHz repetition rate Ti:sapphire laser,�?? Optics Express 11, 933-938 (2003) <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-8-933">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-8-933</a> [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2: Fig. 3:
 

Multimedia

Multimedia FilesRecommended Software
» Media 1: MOV (730 KB)     
» Media 2: MOV (434 KB)     
» Media 3: MOV (738 KB)     
» Media 4: MOV (280 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited