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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 24 — Dec. 1, 2003
  • pp: 3171–3181

Fiber optic confocal reflectance microscopy: a new real-time technique to view nuclear morphology in cervical squamous epithelium in vivo

Kung-Bin Sung, Rebecca Richards-Kortum, Michele Follen, Anais Malpica, Chen Liang, and Michael R. Descour  »View Author Affiliations


Optics Express, Vol. 11, Issue 24, pp. 3171-3181 (2003)
http://dx.doi.org/10.1364/OE.11.003171


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Abstract

We present a fiber optic confocal reflectance microscope (FCRM) which can be used to image epithelial tissue with sub-cellular resolution in vivo. Confocal images of normal and abnormal appearing cervical tissue were obtained in vivo from eighteen patients undergoing colposcopic examination of the cervix; biopsy specimens were taken from imaged sites. The measured lateral and axial resolutions of the system were 1.6 µm and 3 µm, respectively. Morphologic features, including nuclear size and nuclear-to-cytoplasmic ratio, were extracted from confocal images obtained at various depths beneath the epithelial surface. Image features extracted from confocal images compared well with features extracted from confocal images obtained in vitro and from previous histopathologic studies. This study shows that fiber optic confocal reflectance microscopy can be used to visualize the morphology of cervical epithelium in vivo.

© 2003 Optical Society of America

OCIS Codes
(110.2350) Imaging systems : Fiber optics imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4440) Medical optics and biotechnology : ObGyn
(180.1790) Microscopy : Confocal microscopy

ToC Category:
Research Papers

History
Original Manuscript: October 15, 2003
Revised Manuscript: November 12, 2003
Published: December 1, 2003

Citation
Kung-Bin Sung, Rebecca Richards-Kortum, Michele Follen, Anais Malpica, Chen Liang, and Michael Descour, "Fiber optic confocal reflectance microscopy: a new real-time technique to view nuclear morphology in cervical squamous epithelium in vivo," Opt. Express 11, 3171-3181 (2003)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-11-24-3171


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References

  1. T. Collier, A. Lacy, A. Malpica, M. Follen, and R. Richards-Kortum, �??Near real time confocal microscopy of amelanotic tissue: detection of dysplasia in ex-vivo cervical tissue,�?? Acad. Radiol. 9, 504-512 (2002). [CrossRef] [PubMed]
  2. B. Masters and A. A. Thaer, �??Real-time scanning slit confocal microscopy of the in vivo human cornea,�?? Appl. Opt. 33, 695-701 (1994). [CrossRef] [PubMed]
  3. M. Rajadhyaksha, R. R. Anderson, and R. H. Webb, �??Video-rate confocal scanning laser microscope for imaging human tissues in vivo,�?? Appl. Opt. 38, 2105-2115 (1999). [CrossRef]
  4. P. Corcuff, C. Chaussepied, G. Madry, and C. Hadjur, �??Skin optics revisited by in vivo confocal microscopy: melanin and sun exposure,�?? J. Cosmet. Sci. 52, 91-102 (2001). [PubMed]
  5. W. M. White, M. Rajadhyaksha, S. Gonzalez, R. L. Fabian, and R. R. Anderson, �??Noninvasive imaging of human oral mucosa in vivo by confocal reflectance microscopy,�?? Laryngoscope 109, 1709-1717 (1999). [CrossRef] [PubMed]
  6. M. Gu, J. R. Sheppard, and X. Gan, �??Image formation in a fiber-optical confocal scanning microscope,�?? J. Opt. Soc. Am. A 8, 1755-1761 (1991). [CrossRef]
  7. S. Kimura and T. Wilson, �??Confocal scanning optical microscope using single-mode fiber for signal detection,�?? Appl. Opt. 30, 2143-2150 (1991). [CrossRef] [PubMed]
  8. A. F. Gmitro and D. Aziz, �??Confocal microscopy through a fiber-optic imaging bundle,�?? Opt. Lett. 18, 565-567 (1993). [CrossRef] [PubMed]
  9. W. McLaren, J. Tan, and M. Quinn, �??Detection of cervical neoplasia using non-invasive fibre-optic confocal microscopy,�?? 5th international multidisciplinary congress EUROGIN 2003, 213-217, Paris, France (April 13-16, 2003).
  10. Mauna Kea Technologies, Paris, France. <a href="http://www.maunakeatech.com">http://www.maunakeatech.com</a>
  11. A. K. Dunn, C. Smithpeter, A. J. Welch, and R. Richards-Kortum, �??Sources of contrast in confocal reflectance imaging,�?? Appl. Opt. 35, 3441-3446 (1996). [CrossRef] [PubMed]
  12. R. Drezek, T. Collier, C. Brookner, A. Malpica, R. Lotan, and R. Richards-Kortum, �??Laser scanning confocal microscopy of cervical tissue before and after application of acetic acid,�?? Am. J. Obstet. Gynecol. 182, 1135-1139 (2000). [CrossRef] [PubMed]
  13. K. B. Sung, C. Liang, M. Descour, M. Follen, A. Malpica, and R. Richards-Kortum, �??Near real time in vivo fiber optic confocal microscopy: sub-cellular structure resolved,�?? J. Microsc. 207, 137-145 (2002). [CrossRef] [PubMed]
  14. K. B. Sung, C. Liang, M. Descour, T. Collier, M. Follen, and R. Richards-Kortum, �??Fiber optic confocal reflectance microscope with miniature objective for in vivo imaging of human tissues,�?? IEEE trans. biomed. eng. 49, 1168-1172 (2002). [CrossRef] [PubMed]
  15. D. C. Walker, B. J. Brown, A. D. Blackett, J. Tidy and R. H. Smallwood, �??A study of the morphological parameters of cervical squamous epithelium,�?? Physiological Measurement 24, 121-135 (2003). [CrossRef] [PubMed]
  16. M. K. Silverman, F. M. Golomb, A. W. Kopf, C. M. Grin-Jorgensen, K. A. Vossaert, J. P. Doyle, and M. J. Levenstein, �??Verification of a formula for determination of preexcision surgical margins from fixed-tissue melanoma specimens,�?? J. Am. Acad. Dermatol. 27, 214�??219 (1992). [CrossRef] [PubMed]
  17. T. Collier, D. Arifler, A. Malpica, M. Follen, and R. Richards-Kortum, �??Determination of epithelial tissue scattering coefficient using confocal microscopy,�?? IEEE J. Sel. Top. Quantum Electron. 9, 307-313 (2003). [CrossRef]
  18. D. Arifler, M. Guillaud, A. Carraro, A. Malpica, M. Follen, and R. Richards-Kortum, �??Light scattering from normal and dysplastic cervical cells at different epithelial depths: finite-difference time-domain modeling with a perfectly matched layer boundary condition,�?? J. Biomed. Opt. 8, 484-494 (2003). [CrossRef] [PubMed]

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