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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 25 — Dec. 15, 2003
  • pp: 3377–3384

Multiphoton polarization imaging of the stratum corneum and the dermis in ex-vivo human skin

Yen Sun, Jiunn-Wen Su, Wen Lo, Sun-Jan Lin, Shiou-Hwa Jee, and Chen-Yuan Dong  »View Author Affiliations


Optics Express, Vol. 11, Issue 25, pp. 3377-3384 (2003)
http://dx.doi.org/10.1364/OE.11.003377


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Abstract

Abstract: In this work, we demonstrate the application of multiphoton polarization imaging in resolving the structures in surface stratum corneum and dermal layers of ex-vivo human skin. By varying the excitation and emission polarizations, we characterized the structural features in both Laurdan labeled stratum corneum and dermal fibers. The results presented here have important consequences in bioimaging applications of the skin. Both the mechanics of transdermal drug delivery across the skin and physiological significance of the structural changes of the dermis can be monitored. Our results show that the transition dipoles of Laurdan molecules are preferentially oriented normal to the membrane surface. Furthermore, polarization imaging shows that fibrous structures in the dermis generate emission aligned strongly along the excitation polarization. This work shows that multiphoton polarization imaging can be a powerful method in identifying structural orientations in the skin and other biological structures.

© 2003 Optical Society of America

OCIS Codes
(170.1870) Medical optics and biotechnology : Dermatology
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Research Papers

History
Original Manuscript: October 23, 2003
Revised Manuscript: November 26, 2003
Published: December 15, 2003

Citation
Yen Sun, Jiunn-Wen Su, Wen Lo, Sun-Jan Lin, Shiou-Hwa Jee, and Chen-Yuan Dong, "Multiphoton polarization imaging of the stratum corneum and the dermis in ex-vivo human skin," Opt. Express 11, 3377-3384 (2003)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-11-25-3377


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References

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