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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 25 — Dec. 15, 2003
  • pp: 3473–3484

Determining nuclear morphology using an improved angle-resolved low coherence interferometry system

John W. Pyhtila, Robert N. Graf, and Adam Wax  »View Author Affiliations


Optics Express, Vol. 11, Issue 25, pp. 3473-3484 (2003)
http://dx.doi.org/10.1364/OE.11.003473


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Abstract

We outline the process for determining the morphology of subsurface epithelial cell nuclei using depth-resolved light scattering measurements. The measurements are accomplished using a second generation angle-resolved low coherence interferometry system. The new system greatly improves data acquisition and analysis times compared to the initial prototype system. The calibration of the new system is demonstrated in scattering studies to determine the size distribution of polystyrene microspheres in a turbid sample. The process for determining the size of cell nuclei is discussed by analyzing measurements of basal cells in a sub-surface layer of intact, unstained epithelial tissue.

© 2003 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(290.0290) Scattering : Scattering

ToC Category:
Research Papers

History
Original Manuscript: November 12, 2003
Revised Manuscript: December 5, 2003
Published: December 15, 2003

Citation
John Pyhtila, Robert Graf, and Adam Wax, "Determining nuclear morphology using an improved angle-resolved low coherence interferometry system," Opt. Express 11, 3473-3484 (2003)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-11-25-3473


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References

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