OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 25 — Dec. 15, 2003
  • pp: 3473–3484

Determining nuclear morphology using an improved angle-resolved low coherence interferometry system

John W. Pyhtila, Robert N. Graf, and Adam Wax  »View Author Affiliations

Optics Express, Vol. 11, Issue 25, pp. 3473-3484 (2003)

View Full Text Article

Enhanced HTML    Acrobat PDF (290 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We outline the process for determining the morphology of subsurface epithelial cell nuclei using depth-resolved light scattering measurements. The measurements are accomplished using a second generation angle-resolved low coherence interferometry system. The new system greatly improves data acquisition and analysis times compared to the initial prototype system. The calibration of the new system is demonstrated in scattering studies to determine the size distribution of polystyrene microspheres in a turbid sample. The process for determining the size of cell nuclei is discussed by analyzing measurements of basal cells in a sub-surface layer of intact, unstained epithelial tissue.

© 2003 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(290.0290) Scattering : Scattering

ToC Category:
Research Papers

Original Manuscript: November 12, 2003
Revised Manuscript: December 5, 2003
Published: December 15, 2003

John Pyhtila, Robert Graf, and Adam Wax, "Determining nuclear morphology using an improved angle-resolved low coherence interferometry system," Opt. Express 11, 3473-3484 (2003)

Sort:  Journal  |  Reset  


  1. Backman, V., V. Gopal, M. Kalashnikov, K. Badizadegan, R. Gurjar, A. Wax, I. Georgakoudi, M. Mueller, C.W. Boone, R.R. Dasari, and M.S. Feld, "Measuring cellular structure at submicrometer scale with light scattering spectroscopy," IEEE. J. Sel. Top. Quantum Electron. 7, 887-893 (2001). [CrossRef]
  2. Sokolov, K., J. Galvan, A. Myakov, A. Lacy, R. Lotan, and R. Richards-Kortum, "Realistic three-dimensional epithelial tissue phantoms for biomedical optics," J. Biomed. Opt. 7, 148-156 (2002). [CrossRef] [PubMed]
  3. Mourant, J.R., T.M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J.P. Freyer, "Polarized angular dependent spectroscopy of epithelial cells and epithelial cell nuclei to determine the size scale of scattering structures," J. Biomed. Opt. 7, 378-387 (2002). [CrossRef] [PubMed]
  4. Wax, A., C. Yang, V. Backman, K. Badizadegan, C.W. Boone, R.R. Dasari, and M.S. Feld, "Cell organization and sub-structure measured using angle-resolved low coherence interferometry," Biophys. J. 82, 2256-2264 (2002). [CrossRef] [PubMed]
  5. Backman, V., M.B. Wallace, L.T. Perelman, J.T. Arendt, R. Gurjar, M.G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J.M. Crawford, M. Fitzmaurice, S. Kabani, H.S. Levin, M. Seiler, R.R. Dasari, I. Itzkan, J. Van Dam, and M.S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35-36 (2000). [CrossRef] [PubMed]
  6. Wallace, M.B., L.T. Perelman, V. Backman, J.M. Crawford, M. Fitzmaurice, M. Seiler, K. Badizadegan, S.J. Shields, I. Itzkan, R.R. Dasari, J. Van Dam, and M.S. Feld, "Endoscopic detection of dysplasia in patients with Barrett's esophagus using light-scattering spectroscopy," Gastroenterology 119, 677-682 (2000). [CrossRef] [PubMed]
  7. Lovat, L.B., D. Pickard, M. Novelli, P.M. Ripley, H. Francis, I.J. Bigio, and S.G. Bown, "A novel optical biopsy technique using elastic scattering spectroscopy for dysplasia and cancer in Barrett's esophagus," Gastrointest. Endosc. 51, 4919 (2000).
  8. Wax, A., C.H. Yang, M.G. Muller, R. Nines, C.W. Boone, V.E. Steele, G.D. Stoner, R.R. Dasari, and M.S. Feld, "In situ detection of neoplastic transformation and chemopreventive effects in rat esophagus epithelium using angle-resolved low-coherence interferometry," Cancer Research 63, 3556-3559 (2003). [PubMed]
  9. Wax, A., C. Yang, V. Backman, M. Kalashnikov, R.R. Dasari, and M.S. Feld, "Determination of particle size using the angular distribution of backscattered light as measured with low-coherence interferometry," J. Opt. Soc. Am. A 19, 737-744 (2002). [CrossRef]
  10. Wax, A. and J.E. Thomas, "Optical heterodyne imaging and Wigner phase space distributions," Opt. Lett. 21, 1427-1429 (1996). [CrossRef] [PubMed]
  11. Wax, A., S. Bali, and J.E. Thomas, "Time-resolved phase-space distributions for light backscattered from a disordered medium," Phys. Rev. Lett. 85, 66-69 (2000). [CrossRef] [PubMed]
  12. van de Hulst, H.C., Light scattering by small particles. 1957, New York: Dover Publications.
  13. Fercher, A.F., W. Drexler, C.K. Hitzenberger, and T. Lasser, "Optical coherence tomography �?? principles and applications," Reports on Progress in Physics 66, 239-303 (2003). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited