OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 26 — Dec. 29, 2003
  • pp: 3583–3591

Fast calculation of fluorescence correlation data with asynchronous time-correlated single-photon counting

Michael Wahl, Ingo Gregor, Matthias Patting, and Jörg Enderlein  »View Author Affiliations


Optics Express, Vol. 11, Issue 26, pp. 3583-3591 (2003)
http://dx.doi.org/10.1364/OE.11.003583


View Full Text Article

Enhanced HTML    Acrobat PDF (113 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Fluorescence correlation spectroscopy (FCS) is a powerful spectroscopic technique for studying samples at dilute fluorophore concentrations down to single molecules. The standard way of data acquisition, at such low concentrations, is an asynchronous photon counting mode that generates data only when a photon is detected. A significant problem is how to efficiently convert such asynchronously recorded photon count data into a FCS curve. This problem becomes even more challenging for more complex correlation analysis such as the recently introduced combination of FCS and time-correlated single-photon counting (TCSPC). Here, we present, analyze, and apply an algorithm that is highly efficient and can easily be adapted to arbitrarily complex correlation analysis.

© 2003 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.1790) Microscopy : Confocal microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Research Papers

History
Original Manuscript: November 20, 2003
Revised Manuscript: December 12, 2003
Published: December 29, 2003

Citation
Michael Wahl, Ingo Gregor, Mattias Patting, and Jörg Enderlein, "Fast calculation of fluorescence correlation data with asynchronous time-correlated single-photon counting," Opt. Express 11, 3583-3591 (2003)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-11-26-3583


Sort:  Journal  |  Reset  

References

  1. C. Zander, J. Enderlein, R.A. Keller (Eds.) Single-Molecule Detection in Solution - Methods and Applications (VCH-Wiley, Berlin/New York, 2002). [CrossRef]
  2. W. Becker, H. Hickl, C. Zander, K.H. Drexhage, M. Sauer, S. Siebert, J. Wolfrum �??Time-resolved detection and identification of single analyte molecules in microcapillaries by time-correlated single-photon counting (TCSPC),�?? Rev. Sci. Instrum. 70, 1835-41 (1999). [CrossRef]
  3. M. Böhmer, F. Pampaloni, M. Wahl, H.J. Rahn, R. Erdmann, J. Enderlein �??Advanced Time-Resolved Confocal Scanning Device For Ultrasensitive Fluorescence Detection,�?? Rev. Sci. Instrum. 72, 4145-52 (2001). [CrossRef]
  4. C. Eggeling, S. Berger, L. Brand, J.R. Fries, J. Schaffer, A. Volkmer, C.A.M. Seidel �??Data registration and selective single-molecule anaylsis using mulit-parameter fluorescence detection,�?? J. Biotechnol. 86, 163-80 (2001). [CrossRef] [PubMed]
  5. M. Böhmer, J. Enderlein �??Single molecule detection on surfaces with the confocal laser scanning microscope,�?? in ref.[1], pp.145-83.
  6. R. Rigler, E. Elson (Eds.) Fluorescence Correlation Spectroscopy (Springer, New York/Berlin, 2001). [CrossRef]
  7. D.C. Lamb, A. Schenk, C. Röcker, C. Scalfi-Happ, G.U. Nienhaus �??Sensitivity Enhancement in Fluorescence Correlation Spectroscopy of Multiple Species Using Time-Gated Detection,�?? Biophys. J. 79, 1129-38 (2000). [CrossRef] [PubMed]
  8. M. Böhmer, M. Wahl, H.J. Rahn, R. Erdmann, J. Enderlein �??Time-resolved fluorescence correlation spectroscopy,�?? Chem. Phys. Lett. 353, 439-45 (2002). [CrossRef]
  9. J.S. Eid, J.D. Müller, E. Gratton �??Data acquisition card for fluctuation correlation spectroscopy allowing full access to the detected photon sequence,�?? Rev. Sci. Instrum. 71, 361-8 (2000). [CrossRef]
  10. D. Magatti, F. Ferri �??25 ns software correlator for photon and fluorescence correlation spectroscopy,�?? Rev. Sci. Instrum. 74, 1135-44 (2003). [CrossRef]
  11. K. Schätzel �??Correlation techniques in dynamic light scattering,�?? Appl. Phys. B. 42, 193-213 (1987). [CrossRef]
  12. K. Schätzel, M. Drewel, S. Stimac �??Photon correlation measurements at large lag times: Improving statistical accuracy,�?? J. Mod. Opt. 35, 711-8 (1988). [CrossRef]
  13. P. Schwille, F.J. Meyer-Almes, R. Rigler �??Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution,�?? Biophys. J. 72, 1878-86 (1997). [CrossRef] [PubMed]
  14. M. Höbel, J. Ricka �??Dead-time and afterpulsing correction in multiphoton timing with nonideal detectors,�?? Rev. Sci. Instrum. 65, 2326-36 (1994). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited