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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 26 — Dec. 29, 2003
  • pp: 3583–3591

Fast calculation of fluorescence correlation data with asynchronous time-correlated single-photon counting

Michael Wahl, Ingo Gregor, Matthias Patting, and Jörg Enderlein  »View Author Affiliations

Optics Express, Vol. 11, Issue 26, pp. 3583-3591 (2003)

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Fluorescence correlation spectroscopy (FCS) is a powerful spectroscopic technique for studying samples at dilute fluorophore concentrations down to single molecules. The standard way of data acquisition, at such low concentrations, is an asynchronous photon counting mode that generates data only when a photon is detected. A significant problem is how to efficiently convert such asynchronously recorded photon count data into a FCS curve. This problem becomes even more challenging for more complex correlation analysis such as the recently introduced combination of FCS and time-correlated single-photon counting (TCSPC). Here, we present, analyze, and apply an algorithm that is highly efficient and can easily be adapted to arbitrarily complex correlation analysis.

© 2003 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.1790) Microscopy : Confocal microscopy
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Research Papers

Original Manuscript: November 20, 2003
Revised Manuscript: December 12, 2003
Published: December 29, 2003

Michael Wahl, Ingo Gregor, Mattias Patting, and Jörg Enderlein, "Fast calculation of fluorescence correlation data with asynchronous time-correlated single-photon counting," Opt. Express 11, 3583-3591 (2003)

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