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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 1 — Jan. 12, 2004
  • pp: 208–219

Laser-based mid-infrared reflectance imaging of biological tissues

Bujin Guo, Y. Wang, C. Peng, H. Zhang, G. Luo, H. Le, C. Gmachl, D. Sivco, M. Peabody, and A. Cho  »View Author Affiliations


Optics Express, Vol. 12, Issue 1, pp. 208-219 (2004)
http://dx.doi.org/10.1364/OPEX.12.000208


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Abstract

Mid-infrared (MIR) (3-12 um) spectral imaging is a power analytical tool, but difficult in the back-reflectance mode for in-vivo diagnostics. Feasibility of MIR back-reflectance imaging is demonstrated using MIR semiconductor lasers. Transmittance through 500-µm thick films of water and blood showed a capability to resolve more than 6-OD signal dynamic range. Reflectance scanning imaging through a 150-µm thick film of blood showed negligible scattering effect, indicating the feasibility of optical coherent imaging. The result of coherent imaging of a plant leaf shows a MIR sub-surface image that would not be visible in white light. With two wavelengths, a similar result for a chicken skin subcutaneous tissue at different focal depths was obtained, showing blood vessels beneath a lipid layer. These results suggest that advanced multilaser wavelength systems in the fingerprint spectral region can be a useful tool for in-vivo spectral imaging in biomedical research and diagnostic applications.

© 2004 Optical Society of America

OCIS Codes
(110.3080) Imaging systems : Infrared imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Research Papers

History
Original Manuscript: September 23, 2003
Revised Manuscript: January 6, 2004
Published: January 12, 2004

Citation
Bujin Guo, Y. Wang, C. Peng, H. Zhang, G. Luo, H. Le, C. Gmachl, D. Sivco, M. Peabody, and A. Cho, "Laser-based mid-infrared reflectance imaging of biological tissues," Opt. Express 12, 208-219 (2004)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-12-1-208


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