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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 1 — Jan. 12, 2004
  • pp: 208–219

Laser-based mid-infrared reflectance imaging of biological tissues

Bujin Guo, Y. Wang, C. Peng, H. Zhang, G. Luo, H. Le, C. Gmachl, D. Sivco, M. Peabody, and A. Cho  »View Author Affiliations

Optics Express, Vol. 12, Issue 1, pp. 208-219 (2004)

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Mid-infrared (MIR) (3-12 um) spectral imaging is a power analytical tool, but difficult in the back-reflectance mode for in-vivo diagnostics. Feasibility of MIR back-reflectance imaging is demonstrated using MIR semiconductor lasers. Transmittance through 500-µm thick films of water and blood showed a capability to resolve more than 6-OD signal dynamic range. Reflectance scanning imaging through a 150-µm thick film of blood showed negligible scattering effect, indicating the feasibility of optical coherent imaging. The result of coherent imaging of a plant leaf shows a MIR sub-surface image that would not be visible in white light. With two wavelengths, a similar result for a chicken skin subcutaneous tissue at different focal depths was obtained, showing blood vessels beneath a lipid layer. These results suggest that advanced multilaser wavelength systems in the fingerprint spectral region can be a useful tool for in-vivo spectral imaging in biomedical research and diagnostic applications.

© 2004 Optical Society of America

OCIS Codes
(110.3080) Imaging systems : Infrared imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Research Papers

Original Manuscript: September 23, 2003
Revised Manuscript: January 6, 2004
Published: January 12, 2004

Bujin Guo, Y. Wang, C. Peng, H. Zhang, G. Luo, H. Le, C. Gmachl, D. Sivco, M. Peabody, and A. Cho, "Laser-based mid-infrared reflectance imaging of biological tissues," Opt. Express 12, 208-219 (2004)

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  1. R.G. Messerschmidt, M.A. Harthcock, eds. Infrared Microspectroscopy: Theory and applications. (Marcel Dekker, New York, 1988).
  2. H.H. Mantsch, and D. Chapman, eds. Infrared Spectroscopy of Biomolecules. (Wiley-Liss, New York, 1996).
  3. P. J. Treado and M. D. Morris, �??Infrared and Raman Spectroscopic Imaging,�?? Appl. Spectrosc. Rev. 29, 1 (1994).
  4. J. L. Koenig, S-Q. Wang, and R. Bhargava, �??FTIR Images,�?? Analytical Chemistry July 1, 360A (2001).
  5. E. N. Lewis, I. W. Levin, P. J. Treado, U.S. Patent 5,528,368 (1996).
  6. P. Colarusso, L. H. Kidder, I. W. Levin, J. C. Fraser, J. F. Arens, E. N. Lewis, �??Infrared Spectroscopic Imaging: From Planetary to Cellular System,�?? Appl. Spectrosc. 52, 106A�??120A (1998).
  7. R. A. Crocombe, N. Wright, D. L. Drapcho, W. J. McCarthy, P. Bhandare, E. Y. Jiang, �??FT-IR spectroscopic imaging in the infrared 'fingerprint' region using an MCT array detector,�?? Microsc. Microanal. 3, Supplement 2, 863�??864 (1997).
  8. N.G. Jamin, P. Dumas, J. Moncuit, W.H. Fridman, J.L. Teillaud, G.L. Carr, and G.P. Williams, �??Highly resolved chemical imaging of living cells by using synchrotron infrared microspectrometry,�?? Proc. Natl. Acad. Sci. USA 95, 4837�??40, (1998).
  9. Bujin Guo, Yi Wang, Chuan Peng, Guipeng Luo, Han Q. Le, �??Multi-wavelength mid-infrared microspectral imaging using semiconductor lasers,�?? Appl. Spectrosc. 57, 811-822 (2003). [CrossRef]
  10. M. Diem, S. Boydston-White, and L. Chirboga, �??Infrared Spectroscopy of Cells and Tissues: Shining Light onto a Novel Subject,�?? Appl. Spectrosc. 53, 148A (2003). [CrossRef]
  11. M. Mehrubeoglu, N. Kehtarnavaz, G. Marquez, M. Duvic and L.H.V. Wang, �??Skin lesion classification using oblique-incidence diffuse reflectance spectroscopic imaging,�?? Appl. Opt. 41, 182-192 (2003).
  12. Y. Chen, X. Intes, D.R. Tailor, R.R. Regatte, H. Ma, V. Ntziachristos, J.S. Leigh, R. Reddy, B. Chance, �??Probing rat brain oxygenation with near-infrared spectroscopy (NIRS) and magnetic resonance imaging (MRI),�?? Adv. Exp. Med. Biol. 510, 199-204, (2003).
  13. M.G. Sowa, L. Leonardi, J.R. Payette, J.S. Fish, and H.H. Mantsch, �??Near infrared spectroscopic assessment of hemodynamic changes in the early post-burn period,�?? Burns 27, 241-249 (2001). [CrossRef]
  14. P.R. Moreno, J.E. Muller, �??Detection of high-risk atherosclerotic coronary plaques by intravascular spectroscopy,�?? J. Interv. Cardiol. 16, 243 (2003). [CrossRef]
  15. J. Wang, Y.J. Geng, B. Guo, T. Klima, B.N. Lal, J.T. Willerson, W. Casscells, �??Near-infrared spectroscopic characterization of human advanced atherosclerotic plaques,�?? J. Am. Coll. Cardiol. 39, 1305 (2002). [CrossRef]
  16. P.R. Griffiths and J.M. Olinger, �??Continuum Theories of Diffuse Reflectance�?? in Handbook of Vibrational Spectroscopy, J.M. Chalmers and P.R. Griffiths, ed., pp 1125-1138, Volume II, (John Wiley and Sons, Ltd., UK, 2002).
  17. D.J. Dahm and K.D. Dahm, �??Discontinuum Theory of Diffuse Reflectance,�?? in Handbook of Vibrational Spectroscopy, J.M. Chalmers and P.R. Griffiths, ed., pp1140-1153, Volume II, (John Wiley and Sons, Ltd., UK, 2002).
  18. D.M. Wieliczka, S.S. Weng, M.R. Querry, �??Wedge shaped cell for highly absorbent liquids-infrared optical-constants of water,�?? Appl. Opt. 28, 1714-1719 (1989).
  19. A.L. McKenzie, �??Physics of Thermal Processes in Laser-Tissue Interaction,�?? Phys. Med. Biol. 35, 1175-1209 (1990). [CrossRef]
  20. W.K. Purves, G.H. Orians, H.C. Heller, and D. Sadava, Life, The Science of Biology, 5th Ed. (Sinauer Associates, W.H. Frreeman and Company, 1998).
  21. J. Dingle, �??Manufacturing leather from chicken skin,�?? RIRDC Publication No 01/154, Project No. UQ-24E, (Rural Industries Research and Development Corporation, 2001).
  22. S.L. Jacques, J.R. Roman, and K. Lee, �??Lasers in Imaging Superficial Tissues with Polarized Light,�?? Surgery and Medicine 26, 119�??129 (2000).
  23. S.G. Demos and R.R. Alfano, �??Optical polarization imaging,�?? Appl. Opt. 36, 150-55 (1997).
  24. G. Luo, C, Peng, H.-Q. Le, S.-S. Pei, H. Lee, W.-Y. Hwang, B. and J. Zheng, �??Broadly Wavelength-Tunable External Cavity Mid-Infrared Quantum Cascade Lasers,�?? IEEE J. Quantum Electron. 38, 931 (2002).
  25. Chuan Peng, Guipeng Luo, Han Q. Le, �??Broadband, Continuous, and Fine-Tune Properties of External-Cavity Thermoelectric-Stabilized Mid-infrared Quantum-Cascade Lasers,�?? Appl. Opt. 42, 4877-4882 (2003).

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