OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 1 — Jan. 12, 2004
  • pp: 84–89

Optimization of multi-photon event discrimination levels using Poisson statistics

Jori M. Soukka, Arho Virkki, Pekka E. Hänninen, and Juhani T. Soini  »View Author Affiliations


Optics Express, Vol. 12, Issue 1, pp. 84-89 (2004)
http://dx.doi.org/10.1364/OPEX.12.000084


View Full Text Article

Enhanced HTML    Acrobat PDF (94 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

In applications where random multi-photon events must be distinguishable from the background, detection of the signals must be based on either analog current measurement or photon counting and multi-level discrimination of single and multi-photon events. In this paper a novel method for optimizing photomultiplier (PMT) pulse discrimination levels in single-and multi-photon counting is demonstrated. This calibration method is based on detection of photon events in coincidence to short laser pulses. The procedure takes advantage of Poisson statistics of single- and multi-photon signals and it is applicable to automatic calibration of photon counting devices on production line. Results obtained with a channel photomultiplier (CPM) are shown. By use of three parallel discriminators and setting the discriminator levels according to the described method resulted in a linear response over wide range of random single- and multi-photon signals.

© 2004 Optical Society of America

OCIS Codes
(030.5260) Coherence and statistical optics : Photon counting
(030.5290) Coherence and statistical optics : Photon statistics
(040.5250) Detectors : Photomultipliers

ToC Category:
Research Papers

History
Original Manuscript: December 2, 2003
Revised Manuscript: December 20, 2003
Published: January 12, 2004

Citation
Juri Soukka, Arho Virkki, Pekka Hänninen, and Juhani Soini, "Optimization of multi-photon event discrimination levels using Poisson statistics," Opt. Express 12, 84-89 (2004)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-12-1-84


Sort:  Journal  |  Reset  

References

  1. R. J. Ellis and A. G. Wright: �??Optimal use of photomultipliers for chemiluminescence and bioluminescence applications,�?? Luminescence 14, 11-18 (1999). [CrossRef] [PubMed]
  2. J. T. Soini, J. M. Soukka, E. Soini, and P. E. Hänninen, �??Two-photon excitation microfluorometer for multiplexed single-step bioaffinity assays,�?? Rev. Sci. Instrum. 73, 2680-2685 (2002). [CrossRef]
  3. P. Hänninen, A. Soini, N. Meltola, J. Soini, J. Soukka, and E. Soini, �??A new microvolume technique for bioaffinity assays using two-photon excitation,�?? Nature Biotechnol. 18, 548-550 (2000). [CrossRef]
  4. J. T. Soini, J. M. Soukka, N. J. Meltola, A. E. Soini, E. Soini, and P. E. Hänninen, �??Ultra Sensitive Bioaffinity Assay for Micro Volumes,�?? Single Molecules 1, 203-206 (2000). [CrossRef]
  5. PerkinElmer Optoelectronics data sheet, �??Channel Photomultipliers, Overview and Specifications�?? (PerkinElmer Optoelectronics, 2001), <a href="http://optoelectronics.perkinelmer.com/content/RelatedLinks/cpm_brochure.pdf">http://optoelectronics.perkinelmer.com/content/RelatedLinks/cpm_brochure.pdf</a>.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 
Fig. 4.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited