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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 1 — Jan. 12, 2004
  • pp: 84–89

Optimization of multi-photon event discrimination levels using Poisson statistics

Jori M. Soukka, Arho Virkki, Pekka E. Hänninen, and Juhani T. Soini  »View Author Affiliations

Optics Express, Vol. 12, Issue 1, pp. 84-89 (2004)

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In applications where random multi-photon events must be distinguishable from the background, detection of the signals must be based on either analog current measurement or photon counting and multi-level discrimination of single and multi-photon events. In this paper a novel method for optimizing photomultiplier (PMT) pulse discrimination levels in single-and multi-photon counting is demonstrated. This calibration method is based on detection of photon events in coincidence to short laser pulses. The procedure takes advantage of Poisson statistics of single- and multi-photon signals and it is applicable to automatic calibration of photon counting devices on production line. Results obtained with a channel photomultiplier (CPM) are shown. By use of three parallel discriminators and setting the discriminator levels according to the described method resulted in a linear response over wide range of random single- and multi-photon signals.

© 2004 Optical Society of America

OCIS Codes
(030.5260) Coherence and statistical optics : Photon counting
(030.5290) Coherence and statistical optics : Photon statistics
(040.5250) Detectors : Photomultipliers

ToC Category:
Research Papers

Original Manuscript: December 2, 2003
Revised Manuscript: December 20, 2003
Published: January 12, 2004

Juri Soukka, Arho Virkki, Pekka Hänninen, and Juhani Soini, "Optimization of multi-photon event discrimination levels using Poisson statistics," Opt. Express 12, 84-89 (2004)

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  1. R. J. Ellis and A. G. Wright: �??Optimal use of photomultipliers for chemiluminescence and bioluminescence applications,�?? Luminescence 14, 11-18 (1999). [CrossRef] [PubMed]
  2. J. T. Soini, J. M. Soukka, E. Soini, and P. E. Hänninen, �??Two-photon excitation microfluorometer for multiplexed single-step bioaffinity assays,�?? Rev. Sci. Instrum. 73, 2680-2685 (2002). [CrossRef]
  3. P. Hänninen, A. Soini, N. Meltola, J. Soini, J. Soukka, and E. Soini, �??A new microvolume technique for bioaffinity assays using two-photon excitation,�?? Nature Biotechnol. 18, 548-550 (2000). [CrossRef]
  4. J. T. Soini, J. M. Soukka, N. J. Meltola, A. E. Soini, E. Soini, and P. E. Hänninen, �??Ultra Sensitive Bioaffinity Assay for Micro Volumes,�?? Single Molecules 1, 203-206 (2000). [CrossRef]
  5. PerkinElmer Optoelectronics data sheet, �??Channel Photomultipliers, Overview and Specifications�?? (PerkinElmer Optoelectronics, 2001), <a href="http://optoelectronics.perkinelmer.com/content/RelatedLinks/cpm_brochure.pdf">http://optoelectronics.perkinelmer.com/content/RelatedLinks/cpm_brochure.pdf</a>.

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