Confocal laser scanning fluorescence microscopy with a visible continuum source
Optics Express, Vol. 12, Issue 13, pp. 2844-2850 (2004)
http://dx.doi.org/10.1364/OPEX.12.002844
Enhanced HTML 
Acrobat PDF (151 KB)
Abstract
Confocal laser scanning fluorescence microscopy is demonstrated using a photonic crystal fiber-based excitation source. A 38 cm-long section of photonic crystal fiber is pumped with femtosecond pulses from a Ti:sapphire laser, and the resultant visible continuum is selectively filtered to provide the peak excitation wavelengths required for a range of fluorescently labeled biological tissue.
© 2004 Optical Society of America
OCIS Codes
(060.4370) Fiber optics and optical communications : Nonlinear optics, fibers
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4370) Nonlinear optics : Nonlinear optics, fibers
ToC Category:
Research Papers
History
Original Manuscript: May 13, 2004
Revised Manuscript: June 11, 2004
Published: June 28, 2004
Citation
Gail McConnell, "Confocal laser scanning fluorescence microscopy with a visible continuum source," Opt. Express 12, 2844-2850 (2004)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-12-13-2844
Sort: Journal | Reset
References
- J.B. Pawley Handbook of Biological Confocal Microscopy, 2nd (Plenum Press, New York, 1995). [CrossRef]
- J.M. Girkin, A.I. Ferguson, D.L. Wokosin and A.M. Gurney, ???Confocal microscopy using an InGaN violet laser diode at 406 nm,??? Opt. Express. 7 336-341 (2000); <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-7-10-336">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-7-10-336</a>. [CrossRef] [PubMed]
- D.L. Wokosin, V. Centonze, J.G. White, D.Armstrong, G. Robertson and A.I. Ferguson, ???All-solid-state ultrafast lasers facilitate multiphoton excitation fluorescence imaging,??? IEEE J. Sel. Top. Quantum Electron. 2 1051-1065 (1996). [CrossRef]
- W. Denk, J. Strickler and W.W. Webb, ???Two-photon laser scanning fluorescence microscopy,??? Science 10 73-75 (1990). [CrossRef]
- D.L. Wokosin, J.M. Squirrell, K.W. Eliceiri and J.G. White, ???Optical workstation with concurrent, independent multiphoton imaging and experimental laser microbeam capabilities,??? Rev. Sci. Instrum. 74 193-201 (2003). [CrossRef]
- J.C. Knight, T.A. Birks, P. St. J. Russell and D.M. Atkin, ???All-silica single-mode optical fiber with photonic crystal claddin,g??? Opt. Lett. 21 1547-1549 (1996). [CrossRef] [PubMed]
- T.A. Birks, J.C. Knight and P.St. J. Russell, ???Endlessly single-mode photonic crystal fiber,??? Opt. Lett. 22 961-963 (1997). [CrossRef] [PubMed]
- J..K. Ranka, R.S. Windeler and A.J. Stentz, ???Visible continuum generation in air-silica microstructure optical fibers with anomalous dispersion at 800 nm,??? Opt. Lett. 25 25-27 (2000). [CrossRef]
- J.K. Ranka, R.S. Windeler and A.J. Stentz, ???Optical properties of high-delta air silica microstructure optical fibers,??? Opt. Lett. 25 796-798 (2000). [CrossRef]
- A.V. Husakou and J. Hermann, ???Supercontinuum generation of higher-order solitons by fission in photonic crystal fibers,??? Phys. Rev. Lett. 87 203901-203904 (2001). [CrossRef] [PubMed]
- K.M. Hilligsøe, H.N. Paulsen, J. Thøgersen, S. R. Keiding and J. J. Larsen, ???Initial steps of supercontinuum generation in photonic crystal fibers,??? J. Opt. Soc. Am. B. 20 1887-1893 (2003). [CrossRef]
- G. McConnell and E. Riis, ???Ultra-short pulse compression using photonic crystal fibre,??? App. Phys. B. 78 557-563 (2004). [CrossRef]
- K.L. Corwin, N.R. Newbury, J.M. Dudley, S. Coen, S.A. Diddams, K. Weber and R.S. Windeler, ???Fundamental noise limitations to supercontinuum generation in microstructure fiber,??? Phys. Rev. Lett. 90 113904-1-4 (2003). [CrossRef]
Cited By |
 Alert me when this paper is cited |
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.
Related Journal Articles 
- Superresolving masks for incoherent high-numerical-aperture scanning microscopy in three dimensions (JOSAA)
- Reduced Effects of Spherical Aberration on Penetration Depth Under Two-Photon Excitation (AO)
- Coherent use of opposing lenses for axial resolution increase. II. Power and limitation of nonlinear image restoration (JOSAA)
- Coherent use of opposing lenses for axial resolution increase in fluorescence microscopy. I. Comparative study of concepts (JOSAA)
- Influence of Refractive-Index Mismatch in High-Resolution Three-Dimensional Confocal Microscopy (AO)
Related Conference Papers
- Fluorescence 3D-microscopy with 100 nm resolution
- Deterministically Polarized Fluorescence from Single Dye Molecules Aligned in Liquid Crystal Host
- A 104GHz 328fs soliton pulse train generation through a comb-like dispersion profiled fiber using short high nonlinearity dispersion shifted fibers
- A 104GHz 328fs soliton pulse train generation through a comb-like dispersion profiled fiber using short high nonlinearity dispersion shifted fibers
- Effect of modulational mnstability on broadband wavelength conversion using four wave mixing in optical fiber
- Effect of modulational mnstability on broadband wavelength conversion using four wave mixing in optical fiber
- Commercial inverted microscope retrofitted for confocal scanning with multi-wavelength detection, spatially resolved spectroscopy, and fluorescence anisotropy
« Previous Article | Next Article »
OSA is a member of 