OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 14 — Jul. 12, 2004
  • pp: 3218–3223

Depth-resolved fluorescence spectroscopy reveals layered structure of tissue

Yicong Wu, Peng Xi, Jianan Y. Qu, Tak-Hong Cheung, and Mei-Yung Yu  »View Author Affiliations


Optics Express, Vol. 12, Issue 14, pp. 3218-3223 (2004)
http://dx.doi.org/10.1364/OPEX.12.003218


View Full Text Article

Enhanced HTML    Acrobat PDF (764 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

A confocal fluorescence spectroscopy system is instrumented to study depth-resolved autofluorescence in biological tissue. The system provides the capability of optical sectioning with the maximal detection depth up to 120 µm in the examined tissue samples. It was found that the topmost keratinizing epithelial layer produces strong fluorescence similar to collagen. The fluorescence signal from epithelial tissue between the keratinizing layer and stroma can be well resolved. The study results show that depth-resolved fluorescence spectroscopy has the potential to provide more accurate information for the diagnosis of tissue pathology.

© 2004 Optical Society of America

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Research Papers

History
Original Manuscript: May 25, 2004
Revised Manuscript: June 29, 2004
Published: July 12, 2004

Citation
Yicong Wu, Peng Xi, Jianan Qu, Tak-Hong Cheung, and Mei-Yung Yu, "Depth-resolved fluorescence spectroscopy reveals layered structure of tissue," Opt. Express 12, 3218-3223 (2004)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-12-14-3218


Sort:  Journal  |  Reset  

References

  1. R. Richards-Kortum and E. Sevick-Muraca, ???Quantitative optical spectroscopy for tissue diagnosis,??? Ann. Rev. Phy. Chem. 47 555-606 (1996). [CrossRef]
  2. G. A. Wagnieres, W. M. Star and B. C. Wilson, ???In vivo fluorescence spectroscopy and imaging for oncological applications,??? Photochem. Photobiol. 68, 603-32 (1998). [PubMed]
  3. N. Ramanujam, ???Fluorescence spectroscopy of neoplastic and non-neoplastic tissues,??? Neoplasia 2, 89-117 (2000). [CrossRef] [PubMed]
  4. R. Drezek, C. Brookner, I. Pavlova, I. Boiko, A. Malpica, R. Lotan, M. Follen and R. Richards-Kortum, ???Autofluoresecence microscopy of fresh cervical-tissue sections reveals alterations in tissue biochemistry with dysplasia,??? Photochem. Photobiol. 73, 636-641 (2001). [CrossRef] [PubMed]
  5. M. Kobayashi, K. Shibuya, H. Hoshino, and T. Fujisawa, ???Spectroscopic analysis of the autofluorescence from human bronchus using an ultraviolet laser diode,??? J. Biomed. Opt., 7, 603-608 (2002) [CrossRef] [PubMed]
  6. I. Georgakoudi, B. C. Jacobson, M. G. Muller, E. E. Sheets, K. Badizadegan, D. L. Carr-Locker, C. P. Crum, C. W. Boone, R. R. Dasari, J. V. Dam, and M. S. Feld, ???NAD(P)H and collagen as in vivo quantitative fluorescent biomarkers of epithelial precancerous changes,??? Cancer Res. 62, 682-687 (2002). [PubMed]
  7. N. Ghosh, S. K. Majumder and P. K. Gupta, ???Polarized fluorescence spectroscopy of human tissues,??? Opt. Lett. 22, 2007-2009 (2002). [CrossRef]
  8. M. G. Skala, G. M. Palmer, C. Zhu, Q. Liu, K. M. Vrotsos, C. L. Marshek-stone, A. Gendron-Fitzpatrick, and N. Ramanujam, ???Investigation of fiber-optic probe designs for optical spectroscopic diagnosis of epithelial pre-cancers,??? Lasers Surg. Med. 34, 25-38 (2004). [CrossRef] [PubMed]
  9. C. E. Bigelow, D. L. Conover and T. H. Foster, ???Confocal fluorescence spectroscopy and anisotropy imaging system,??? Opt. Lett. 28, 695-697 (2003). [CrossRef] [PubMed]
  10. G. M. Palmer, C. L. Marshek, K. M. Vrotsos and N. Ramanujam, ???Optimal methods for fluorescence and diffuse reflectance measurements of tissue biopsy samples,??? Lasers Surg. Med. 30, 191-200 (2002). [CrossRef] [PubMed]
  11. R. Drezek K. Sokolov, U. Utzinger, I. Boiko, A. Malpica, M. Follen, and R. Richards-Kortum, ???Understanding the contribution of NADH and collagen to cervical tissue fluorescence spectra: modeling, measurements and implications,??? J. Biomed. Opt. 6, 385-396 (2001). [CrossRef] [PubMed]
  12. J. A. Kiernan, Histological and histochemical methods: theory and practice (Butterworth Heinemann, 1999), Chap. 8

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited