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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 14 — Jul. 12, 2004
  • pp: 3218–3223

Depth-resolved fluorescence spectroscopy reveals layered structure of tissue

Yicong Wu, Peng Xi, Jianan Y. Qu, Tak-Hong Cheung, and Mei-Yung Yu  »View Author Affiliations

Optics Express, Vol. 12, Issue 14, pp. 3218-3223 (2004)

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A confocal fluorescence spectroscopy system is instrumented to study depth-resolved autofluorescence in biological tissue. The system provides the capability of optical sectioning with the maximal detection depth up to 120 µm in the examined tissue samples. It was found that the topmost keratinizing epithelial layer produces strong fluorescence similar to collagen. The fluorescence signal from epithelial tissue between the keratinizing layer and stroma can be well resolved. The study results show that depth-resolved fluorescence spectroscopy has the potential to provide more accurate information for the diagnosis of tissue pathology.

© 2004 Optical Society of America

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Research Papers

Original Manuscript: May 25, 2004
Revised Manuscript: June 29, 2004
Published: July 12, 2004

Yicong Wu, Peng Xi, Jianan Qu, Tak-Hong Cheung, and Mei-Yung Yu, "Depth-resolved fluorescence spectroscopy reveals layered structure of tissue," Opt. Express 12, 3218-3223 (2004)

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