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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 18 — Sep. 6, 2004
  • pp: 4203–4213

Femtosecond laser disruption of subcellular organelles in a living cell

Wataru Watanabe, Naomi Arakawa, Sachihiro Matsunaga, Tsunehito Higashi, Kiichi Fukui, Keisuke Isobe, and Kazuyoshi Itoh  »View Author Affiliations

Optics Express, Vol. 12, Issue 18, pp. 4203-4213 (2004)

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Subcellular organelles in living cells were inactivated by tightly focusing femtosecond laser pulses inside the cells. Photodisruption of a mitochondrion in living cells was experimentally confirmed by stacking three-dimensional confocal images and by restaining of organelles. The viability of the cells after femtosecond laser irradiation was ascertained by impermeability of propidium iodide as well as by the presence of cytoplasmic streaming.

© 2004 Optical Society of America

OCIS Codes
(140.3440) Lasers and laser optics : Laser-induced breakdown
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(320.7110) Ultrafast optics : Ultrafast nonlinear optics

ToC Category:
Research Papers

Original Manuscript: August 4, 2004
Revised Manuscript: August 20, 2004
Published: September 6, 2004

Wataru Watanabe, Naomi Arakawa, Sachihiro Matsunaga, Tsunehito Higashi, Kiichi Fukui, Keisuke Isobe, and Kazuyoshi Itoh, "Femtosecond laser disruption of subcellular organelles in a living cell," Opt. Express 12, 4203-4213 (2004)

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