OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 18 — Sep. 6, 2004
  • pp: 4246–4254

Supercritical angle fluorescence (SAF) microscopy

Thomas Ruckstuhl and Dorinel Verdes  »View Author Affiliations


Optics Express, Vol. 12, Issue 18, pp. 4246-4254 (2004)
http://dx.doi.org/10.1364/OPEX.12.004246


View Full Text Article

Enhanced HTML    Acrobat PDF (1051 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We explore a new confocal microscope for the detection of surface-generated fluorescence. The instrument is designed for high resolution imaging as well as for the readout of large biochips. Special feature is the separated collection of two different fluorescence emission modes. One optical path covers the emission into the glass at low surface angles, the other captures high angles, exceeding the critical angle of the water/glass interface. Due to the collection of the supercritical angle fluorescence (SAF) the confocal detection volume is strictly confined to the interface, whereas the low angles collect much deeper from the aqueous analyte solution. Hence the system can deliver information about surface-bound and unbound fraction of fluorescent analyte simultaneously.

© 2004 Optical Society of America

OCIS Codes
(080.2740) Geometric optics : Geometric optical design
(180.1790) Microscopy : Confocal microscopy
(260.6970) Physical optics : Total internal reflection
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Research Papers

History
Original Manuscript: July 15, 2004
Revised Manuscript: August 24, 2004
Published: September 6, 2004

Citation
Thomas Ruckstuhl and Dorinel Verdes, "Supercritical angle fluorescence (SAF) microscopy," Opt. Express 12, 4246-4254 (2004)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-12-18-4246


Sort:  Journal  |  Reset  

References

  1. D. Axelrod, �??Total internal reflection fluorescence microscopy,�?? in Methods in Cellular Imaging, Ammasi Periasamy, ed. (Oxford University Press, New York, 2001), pp. 362-380.
  2. R. L. Hansen and J. M. Harris, �??Total internal reflection fluorescence correlation spectroscopy for counting molecules at solid/liquid interfaces,�?? Anal. Chem. 70, 2565-2575 (1998). [CrossRef] [PubMed]
  3. T. E. Starr and N. L. Thompson, �??Total internal reflection with correlation spectroscopy: Combined surface reaction and solution diffusion,�?? Biophys. J. 80, 1575-1584 (2001). [CrossRef] [PubMed]
  4. A. M. Lieto, R. C. Cush, N. L. Thompson, �??Ligand-receptor kinetics measured by total internal reflection with fluorescence correlation spectroscopy,�?? Biophys. J. 85, 3294-3302 (2003) [CrossRef] [PubMed]
  5. M. J. Levene, J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, W. W. Webb, �??Zero-mode waveguides for single-molecule analysis at high concentrations,�?? Science 299, 682-686 (2003). [CrossRef] [PubMed]
  6. T. Ruckstuhl and S. Seeger, WO 9946596 (1999).
  7. J. Enderlein, T. Ruckstuhl, S. Seeger, �??Highly efficient optical detection of surface-generated fluorescence,�?? Appl. Opt. 38, 724-732 (1999). [CrossRef]
  8. T. Ruckstuhl, J. Enderlein, S. Jung, S. Seeger, �??Forbidden light detection from single molecules,�?? Anal. Chem. 72, 2117-2123 (2000). [CrossRef] [PubMed]
  9. T. Ruckstuhl, M. Rankl, S. Seeger, �??Highly sensitive biosensing using a supercritical angle fluorescence (SAF) instrument,�?? Biosens. Bioelectron. 18, 1193-1199 (2003). [CrossRef] [PubMed]
  10. A. Krieg, S. Laib, T. Ruckstuhl, S. Seeger, �??Real-time detection of nucleotide-incorporation during complimentary DNA strand synthesis,�?? Chem BioChem. 4, 589-592 (2003). [CrossRef] [PubMed]
  11. T. Ruckstuhl and S. Seeger, �??Confocal total-internal-reflection microscopy with a high-aperture parabolic mirror lens,�?? Appl. Opt. 42, 3277-3283 (2003). [CrossRef] [PubMed]
  12. T. Ruckstuhl and S. Seeger, �??Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopy,�?? Opt. Lett. 29, 569-571 (2004). [CrossRef] [PubMed]
  13. T. Ruckstuhl, A. Walser, D. Verdes, S. Seeger, �??Confocal reader for biochip screening and fluorescence microscopy,�?? Biosens. Bioelectron. (to be published).
  14. T. A. Laurence and S. Weiss, �??How to detect weak pairs,�?? Science 299, 667-668 (2003). [CrossRef] [PubMed]
  15. P. Schwille, �??TIR-FCS: Staying at the surface can sometimes be better,�?? Biophys. J. 85, 2783-2784 (2003). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited