Supercritical angle fluorescence (SAF) microscopy
Optics Express, Vol. 12, Issue 18, pp. 4246-4254 (2004)
http://dx.doi.org/10.1364/OPEX.12.004246
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Abstract
We explore a new confocal microscope for the detection of surface-generated fluorescence. The instrument is designed for high resolution imaging as well as for the readout of large biochips. Special feature is the separated collection of two different fluorescence emission modes. One optical path covers the emission into the glass at low surface angles, the other captures high angles, exceeding the critical angle of the water/glass interface. Due to the collection of the supercritical angle fluorescence (SAF) the confocal detection volume is strictly confined to the interface, whereas the low angles collect much deeper from the aqueous analyte solution. Hence the system can deliver information about surface-bound and unbound fraction of fluorescent analyte simultaneously.
© 2004 Optical Society of America
OCIS Codes
(080.2740) Geometric optics : Geometric optical design
(180.1790) Microscopy : Confocal microscopy
(260.6970) Physical optics : Total internal reflection
(300.2530) Spectroscopy : Fluorescence, laser-induced
ToC Category:
Research Papers
History
Original Manuscript: July 15, 2004
Revised Manuscript: August 24, 2004
Published: September 6, 2004
Citation
Thomas Ruckstuhl and Dorinel Verdes, "Supercritical angle fluorescence (SAF) microscopy," Opt. Express 12, 4246-4254 (2004)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-12-18-4246
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