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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 19 — Sep. 20, 2004
  • pp: 4457–4466

Multiple UV wavelength excitation and fluorescence of bioaerosols

Vasanthi Sivaprakasam, Alan L. Huston, Cathy Scotto, and Jay D. Eversole  »View Author Affiliations

Optics Express, Vol. 12, Issue 19, pp. 4457-4466 (2004)

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A two-wavelength laser-induced fluorescence technique is described for detecting and classifying biological aerosols. Single aerosols, smaller than 10 μm, are interrogated with 266 nm and 355 nm laser pulses separated in time by 400 ns. Fluorescence signals excited by these pulses are detected in three broad spectral bands centered at 350 nm, 450 nm and 550 nm. The results indicate that bacterial spores, vegetative bacterial cells and proteins can be differentiated based on the two wavelength excitation approach. Estimates of the fluorescence cross sections for 16 bioaerosol simulants and interferents are presented.

© 2004 Optical Society of America

OCIS Codes
(290.5850) Scattering : Scattering, particles
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Research Papers

Original Manuscript: July 14, 2004
Revised Manuscript: September 7, 2004
Published: September 20, 2004

Vasanthi Sivaprakasam, Alan Huston, Cathy Scotto, and Jay Eversole, "Multiple UV wavelength excitation and fluorescence of bioaerosols," Opt. Express 12, 4457-4466 (2004)

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