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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 19 — Sep. 20, 2004
  • pp: 4496–4510

Noninvasive, optical detection of diabetes: model studies with porcine skin

E.L. Hull, M.N. Ediger, A.H.T. Unione, E.K. Deemer, M.L. Stroman, and J.W Baynes  »View Author Affiliations

Optics Express, Vol. 12, Issue 19, pp. 4496-4510 (2004)

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An in vitro study was performed to evaluate noninvasive spectroscopic measurement of advanced glycation endproducts (AGEs) in skin collagen. A porcine dermis preparation was incubated in solutions simulating normal and hyperglycemic conditions. The AGEs kinetics of increase were determined by HPLC and GC/MS assays, and compared to near-infrared (NIR) and ultraviolet/visible fluorescence skin spectra. Multivariate analysis indicated that, although NIR did not discriminate between collagen samples exposed to different glucose concentrations, fluorescence changes were readily detected and correlated strongly with skin concentration of AGEs. These results suggest that measurement of skin AGEs by fluorescence spectroscopy may be useful for detection and diagnosis of type II diabetes.

© 2004 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.1580) Medical optics and biotechnology : Chemometrics
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Research Papers

Original Manuscript: August 10, 2004
Revised Manuscript: September 9, 2004
Published: September 20, 2004

E. Hull, M. Ediger, A. Unione, E. Deemer, M. Stroman, and J. Baynes, "Noninvasive, optical detection of diabetes: model studies with porcine skin," Opt. Express 12, 4496-4510 (2004)

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