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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 2 — Jan. 26, 2004
  • pp: 331–341

Nonlinear optical contrast enhancement for optical coherence tomography

Claudio Vinegoni, Jeremy S. Bredfeldt, Daniel L. Marks, and Stephen A. Boppart  »View Author Affiliations

Optics Express, Vol. 12, Issue 2, pp. 331-341 (2004)

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We present a new interferometric technique for measuring Coherent Anti-Stokes Raman Scattering (CARS) and Second Harmonic Generation (SHG) signals. Heterodyne detection is employed to increase the sensitivity in both CARS and SHG signal detection, which can also be extended to different coherent processes. The exploitation of the mentioned optical nonlinearities for molecular contrast enhancement in Optical Coherence Tomography (OCT) is presented.

© 2004 Optical Society of America

OCIS Codes
(040.2840) Detectors : Heterodyne
(110.4500) Imaging systems : Optical coherence tomography
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4410) Nonlinear optics : Nonlinear optics, parametric processes
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:
Research Papers

Original Manuscript: December 17, 2003
Revised Manuscript: January 15, 2004
Published: January 26, 2004

Claudio Vinegoni, Jeremy Bredfeldt, Daniel Marks, and Stephen Boppart, "Nonlinear optical contrast enhancement for optical coherence tomography," Opt. Express 12, 331-341 (2004)

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  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafto, and J. G. Fujimoto, �??Optical coherence tomography,�?? Science 254, 1178�??1181 (1991). [CrossRef] [PubMed]
  2. S. A. Boppart, B. E. Bouma, C. Pitris, J. F. Southern, M. E. Brezinski, and J. G. Fujimoto, �??In vivo cellular optical coherence tomography imaging,�?? Nat. Med. 4, 861�??5 (1998). [CrossRef] [PubMed]
  3. Y. Wang, Y. Zhao, Z. Chen, R. S. Windeler, and J. Nelson, �??Ultrahigh-resolution optical coherence tomography by broadband continuum generation from a photonic crystal fiber,�?? Opt. Lett. 28, 182�??184 (2003). [CrossRef] [PubMed]
  4. J. G. Fujimoto, �??Optical coherence tomography for ultrahigh resolution in vivo imaging,�?? Nat. Biotechnol. 21, 1361�??1367 (2003). [CrossRef] [PubMed]
  5. T. M. Lee, A. L. Oldenburg, S. Sitafalwalla, D. L. Marks, W. Luo, F. J.-J. Toublan, K. S. Suslick, and S. A. Boppart, �??Engineered microsphere contrast agents for optical coherence tomography,�?? Opt. Lett. 28, 1546�??1548 (2003). [CrossRef] [PubMed]
  6. U. Morgner, W. Drexler, F. Kartner, X. Li, C. Pitris, E. Ippen, and J. Fujimoto, �??Spectroscopic optical coherence tomography,�?? Opt. Lett. 25, 111�??13 (2000). [CrossRef]
  7. K. Divakar Rao, M. A. Choma, S. Yazdanfar, A. M. Rollins, and J. A. Izatt, �??Molecular contrast in optical coherence tomography by use of a pump-probe technique,�?? Opt. Lett. 28, 340�??2 (2003). [CrossRef] [PubMed]
  8. J. K. Barton, J. B. Hoying, and C. J. Sullivan, �??Use of microbubbles as an optical coherence tomography contrast agent,�?? Acad. Radiol. 9, S52�??5 (2002). [CrossRef] [PubMed]
  9. R. K. Chang, J. Ducuing, and N. Bloembergen, �??Relative phase measurement between fundamental and second-harmonic light,�?? Phys. Rev. Lett. 15, 6�??9 (1965). [CrossRef]
  10. R. Stolle, G. Marowsky, E. Schwarzberg, and G. Berkovic �??Phase measurements in nonlinear optics,�?? Appl. Phys. B 63, 491�??8 (1996).
  11. G. Marowsky, and G. Luepke �??CARS-background suppression by phase-controlled nonlinear interferometry,�?? Appl. Phys. B 51, 49�??51 (1990). [CrossRef]
  12. J.W. Hahn, and E.S. Lee �??Measurement of nonresonant third-order susceptibilities of various gases by the nonlinear interferometric technique,�?? JOSA B 12, 1021�??27 (1995). [CrossRef]
  13. A. Zumbusch, G. Holtorn, and X. Sunney Xie, �??Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering,�?? Phys. Rev. Lett. 82, 4142�??5 (1999). [CrossRef]
  14. W. Dermtroeder, Laser Spectroscopy (Springer, 1998).
  15. J.-X. Cheng, A. Volkmer, and X. Xie, �??Theoretical and experimental characterization of coherent anti-Stokes Raman scattering microscopy,�?? J. Opt. Soc. Am. B 19, 1363�??75 (2002). [CrossRef]
  16. M. Duncan, J. Reintjes, and T. Manuccia, �??Scanning coherent anti-Stokes Raman microscope,�?? Opt. Lett. 7, 350�??2 (1982). [CrossRef] [PubMed]
  17. G. W. Wurpel, J. M. Schins, and M. Muller, �??Chemical specificity in three-dimensional imaging with multiplex coherent anti-Stokes Raman scattering microscopy,�?? Opt. Lett. 27, 1093�??1095 (2002). [CrossRef]
  18. M. Hashimoto, T. Araki, and S. Kawata, �??Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration,�?? Opt. Lett. 25, 1768�??1770 (2000). [CrossRef]
  19. J.-X. Cheng, Y. Kevin Jia, G. Zheng, and X. Xie, �??Laser�??scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology,�?? Biophys. Journal 83, 502�??9 (2002). [CrossRef]
  20. D. L. Marks and S. A. Boppart, �??Nonlinear interferometric vibrational imaging: theory and simulations,�?? In Press, Phys. Rev. Lett.; LANL E�??print arXive <a href="http://www.arxiv.org/abs/physics/0311071 (2003)">http://www.arxiv.org/abs/physics/0311071 (2003)</a>.
  21. D. L. Marks, J. Bredfeldt, S. Hambir, D. Dlott, B. Kitchell, M. Gruebele, and S. A. Boppart, �??Molecular species sensitive optical coherence tomography using coherent anti-Stokes Raman scattering spectroscopy,�?? in Coherence domain optical methods and OCT in biomedicine VII, V.V. Tuchin, J.A. Izatt, and J.G. Fujimoto, eds., Proc. SPIE 4956, 9�??13 (2003).
  22. J. Bredfeldt, D. L. Marks, C. Vinegoni, S. Hambir, and S. A. Boppart, �??Coherent anti-Stokes Raman scattering heterodyne interferometry,�?? Submitted; LANL E�??print arXive <a href="http://www.arxiv.org/abs/physics/0311057 (2003)">http://www.arxiv.org/abs/physics/0311057 (2003)</a>.
  23. P. J. Campagnola and L. M. Loew, �??Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms,�?? Nat. Biotechnol. 21, 1356�??1360 (2003). [CrossRef] [PubMed]
  24. P. Stoller, P. M. Celliers, K. M. Reiser, and A. M. Rubenchik, �??Quantitative second-harmonic generation microscopy in collagen,�?? Appl. Optics 42, 5209�??5219 (2003). [CrossRef]
  25. I. Freund and M. Deutsch, �??Connective tissue polarity. Optical second-harmonic microscopy, crossed-beam summation, and small-angle scattering in Rat-tail tendon,�?? Biophys. J. 50, 693�??712 (1986). [CrossRef] [PubMed]
  26. K. Eisenthal, �??Liquid Interfaces Probed by Second-Harmonic and Sum-Frequency Spectroscopy,�?? Chem. Rev. 96, 1343�??60 (1996). [CrossRef] [PubMed]
  27. R. Brown, A. Millard, and P. J. Campagnola, �??Macromolecular structure of cellulose studied by second-harmonic generation imaging microscopy. �?? Opt. Lett. 28, 2207�??2209 (2003). [CrossRef] [PubMed]
  28. J. Squier, M. Muller, G. Brakenhoff, and K. Wilson, �??Third harmonic generation microscopy,�?? Opt. Express 3, 315 �??24 (1998), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-9-315">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-9-315</a>. [CrossRef] [PubMed]

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