OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 21 — Oct. 18, 2004
  • pp: 5178–5190

Polarimetric imaging and blood vessel quantification

Anke Weber, Michael C. Cheney, Quinn Y.J. Smithwick, and Ann E. Elsner  »View Author Affiliations

Optics Express, Vol. 12, Issue 21, pp. 5178-5190 (2004)

View Full Text Article

Enhanced HTML    Acrobat PDF (947 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We applied a polarimetric analysis to retinal imaging, to examine the potential improvement in characterizing blood vessels. To minimize the reflection artifact of the superficial wall of the blood vessel, we computed depolarized light images by removing the polarization retaining light reaching the instrument. These depolarized light images were compared to images from the average of all the light. Michelson contrast was computed for the vessel profiles across arteries and veins, and was higher for the depolarized light images. Depolarized light images provide one step towards improving the characterization of retinal blood vessels.

© 2004 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4470) Medical optics and biotechnology : Ophthalmology
(230.5440) Optical devices : Polarization-selective devices

ToC Category:
Research Papers

Original Manuscript: August 17, 2004
Revised Manuscript: October 6, 2004
Published: October 18, 2004

Anke Weber, Michael Cheney, Quinn Smithwick, and Ann Elsner, "Polarimetric imaging and blood vessel quantification," Opt. Express 12, 5178-5190 (2004)

Sort:  Journal  |  Reset  


  1. B. B. Boycott, J. W. Dowling, �??Organization of the primate retina: Light microscopy,�?? Philosophical Transactions of the Royal Society of London B 255: 109-184 (1969) [CrossRef]
  2. A. E. Elsner, S. A. Burns, J. J. Weiter, F. C. Delori, �??Infrared imaging of subretinal structures in the human ocular fundus,�?? Vis Res 36, 191-205 (1996) [CrossRef] [PubMed]
  3. M. Miura, A. E. Elsner, �??Three dimensional imaging in age-related macular degeneration,�?? Opt Express 9, 436-443 (2001) [CrossRef] [PubMed]
  4. A. E. Elsner, M. Miura, J. B. Stewart, M. B. M. Kairala, S.A. Burns, �?? Novel Algorithm for polarization imaging resulting in improved quantification of retinal blood vessels,�?? Medicine Meets Virtual Reality 11, 59-61 (2003)
  5. S. A. Burns, A. E. Elsner, M. B. Mellem-Kairala, R. B. Simmons, �??Improved contrast of subretinal structures using polarization analysis,�?? Invest. Ophthalmol. Vis. Sci. 44, 4061-8 (2003) [CrossRef] [PubMed]
  6. T.S. Leeson, C.R. Leeson, A.A. Paparo, �??The circulatory system�?? in Text/Atlas of Histology, T.S. Leeson, C.R. Leeson, A.A. Paparo , eds. (Saunders, Philadelphia, PA 1988), pp. 309-327
  7. A.E. Elsner, S.A. Burns, F.C. Delori, R.H. Webb, "Quantitative Reflectometry with the SLO," in Laser Scanning Ophthalmoscopy and Tomography, J. E. Nasemann and R.O.W. Burk, eds. (Quintessenz-Verlag, Muenchen, 1990), pp. 109-121
  8. R.H. Webb and F.C. Delori, �??How we see the retina.�?? in Laser Technology in Ophthalmology, J. Marshall ed., (Kugler & Ghedini Pub. 1988), pp 3-14.
  9. S.A. Burns, S. Marcos, A.E. Elsner, S. Barra, �??Contrast Improvement for Confocal Retinal Imaging Using Phase Correcting Plates,�?? Optics Letters. 27, 400-402 (2002) [CrossRef]
  10. N. Chapman, N.Witt, X. Gao, A.A. Bharath, A.V. Stanton, S.A. Thom, A.D. Hughes, �??Computer algorithms for the automated measurements of retinal arteriolar diameters,�?? Br. J. Ophthalmol. 85,74-79 (2001). [CrossRef] [PubMed]
  11. M. H. Smith, K. R. Denninghoff, A. Lompado, Hillman LW, �??Effect of multiple light paths on retinal vessel oximetry", Appl. Opt. 3, 1183-1193 (2000) [CrossRef]
  12. N. T. Choplin, D. C. Lundy, A. W. Dreher, �?? Differentiating patients with glaucoma from glaucoma suspects and normal subjects by nerve fiber layer assessment with scanning laser polarimetry,�?? Ophthalmology 105, 2068-2076 (1998) [CrossRef] [PubMed]
  13. L. S. Lasdon, R. L. Fox, M. W. Ratner, �??Nonlinear optimization using the generalized reduced gradient method,�?? RAIRO 3, 73-104 (1974)
  14. K. R. Denninghoff, M. H. Smith, M. H. Lompado, L.W. Hillman, �??Retinal venous oxygen saturation and cardiac output during controlled hemorrhage and resuscitation,�?? J. Appl. Physiol. 94, 891-896 (2003) [PubMed]
  15. R. Klein, B. E. K. Klein, S. C. Tomany, T. Y. Wong, �??The relation of retinal microvascular characteristics to age-related eye disease: the Beaver Dam Eye Study,�?? Am. J. Ophthalmol, 137 435-444 (2004) [CrossRef] [PubMed]
  16. Greenfield, R.W. Knighton, W.J. Feuer, J.C. Schiffman, L. Zangwill and R.N. Weinreb, �??Correction for corneal polarization axis improves the discriminating power of scanning laser polarimetry,�?? Am. J. Ophthalmol. 134, 27-33 (2002) [CrossRef] [PubMed]
  17. M. Miura, M.Osako, A. E. Elsner, H. Kajizuka, K. Uamada, M. Usua, �??Birefringence of intraocular lenses,�?? J Cataract Refract Surg. 30, 1549-55 (2004) [CrossRef] [PubMed]
  18. R.A. Chipman �??Polarimetry�?? in The Handbook of Optics, M. Bass, E.W. van Stryland, D.R.Williams, W.I.Wolfe, eds. (McGraw Hill, New York, 1994) pp. 1-27
  19. J. M. Bueno, M.C.W. Campbell, �??Confocal scanning laser ophthalmoscopy improvement by use of Muellermatrix polarimetry,�?? Opt. Lett. 27, 830-832 (2002) [CrossRef]
  20. J. M. Bueno, �??Polarimetry in the human eye using an imaging linear polariscope,�?? J. Opt. A-Pure Appl. Opt. 4, 553-561 (2002) [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Supplementary Material

» Media 1: AVI (479 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited