OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 22 — Nov. 1, 2004
  • pp: 5487–5501

Optical coherence tomography contrast enhancement using spectroscopic analysis with spectral autocorrelation

Desmond C. Adler, Tony H. Ko, Paul R. Herz, and James G. Fujimoto  »View Author Affiliations

Optics Express, Vol. 12, Issue 22, pp. 5487-5501 (2004)

View Full Text Article

Enhanced HTML    Acrobat PDF (5754 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Enhanced tissue contrast in developmental biology specimens is demonstrated in vivo using a new type of spectroscopic optical coherence tomography analysis that is insensitive to spectroscopic noise sources. The technique is based on a statistical analysis of spectral modulation at each image pixel, and provides contrast based on both the intensity of the backscattered light and the distribution of scattering particle sizes. Since the technique does not analyze optical power at absolute wavelengths, it is insensitive to all spectroscopic noise that appears as local Doppler shifts. No exogenous contrast agents or dyes are required, and no additional components are needed to correct for reference arm motion.

© 2004 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Research Papers

Original Manuscript: August 10, 2004
Revised Manuscript: September 11, 2004
Published: November 1, 2004

Desmond Adler, Tony Ko, Paul Herz, and James Fujimoto, "Optical coherence tomography contrast enhancement using spectroscopic analysis with spectral autocorrelation," Opt. Express 12, 5487-5501 (2004)

Sort:  Journal  |  Reset  


  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991) [CrossRef] [PubMed]
  2. U. Morgner, W. Drexler, F. X. Kartner, X. D. Li, C. Pitris, E. P. Ippen, and J. G. Fujimoto, "Spectroscopic optical coherence tomography," Opt. Lett. 25, 111-113 (2000). [CrossRef]
  3. C. Xu, J. Ye, D. Marks, and S. Boppart, "Near infrared dyes as contrast-enhancing agents for spectroscopic optical coherence tomography," Opt. Lett. 29, 1647-1649 (2004) [CrossRef] [PubMed]
  4. C. Yang, L.E.L. McGuckin, J.D. Simon, M.A. Choma, B.E. Applegate, J.A. Izatt, �??Spectral triangulation molecular contrast optical coherence tomography with indocyanine green as the contrast agent,�?? Opt. Lett. 29, 2016-2018 (2004) [CrossRef] [PubMed]
  5. D. L. Faber, E. G. Mik, M. C. G. Aalders, and T. G. van Leeuwen, "Light absorption of (oxy-)hemoglobin assessed by spectroscopic optical coherence tomography," Opt. Lett. 28, 1436-1438 (2003) [CrossRef] [PubMed]
  6. L. T. Perelman, V. Backman, M. Wallace, G. Zonios, R. Manoharan, A. Nusrat, S. Shields, M. Seiler, C. Lima, T. Hamano, I. Itzkan, J. Van Dam, J. M. Crawford, and M. S. Feld, "Observation of periodic fine structure in reflectance from biological tissue: a new technique for measuring nuclear size distribution," Phys. Rev. Lett. 80, 627-630 (1998) [CrossRef]
  7. V. Backman, R. Gurjar, K. Badizadegan, I. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, "Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ," IEEE J. Sel. Top. Quantum Electron. 5, 1019-1026 (1999) [CrossRef]
  8. V. Backman, M. Wallace, L. T. Perelman, J. Arendt, R. Gurjar, M. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35-36 (2000) [CrossRef] [PubMed]
  9. M. Wallace, L. T. Perelman, V. Backman, J. M. Crawford, M. Fitzmaurice, M. Seiler, K. Badizadegan, S. Shields, I. Itzkan, R. R. Dasari, J. Van Dam, and M. S. Feld, "Endoscopic detection of dysplasia in patients with Barrett's esophagus using light-scattering spectroscopy," Gastroenterology 119, 677 (2000) [CrossRef] [PubMed]
  10. V. Backman, V. Gopal, M. Kalashnikov, K. Badizadegan, R. Gurjar, A. Wax, I. Georgakoudi, M. Mueller, C. W. Boone, R. R. Dasari, and M. S. Feld, "Measuring cellular structure at submicrometer scale with light scattering spectroscopy," IEEE J. Sel. Top. Quantum Electron. 7, 887-893 (2001) [CrossRef]
  11. R. Gurjar, V. Backman, K. Badizadegan, R. R. Dasari, I. Itzkan, L. T. Perelman, and M. S. Feld, "Imaging human epithelial properties with polarized light scattering spectroscopy," Nature Medicine 7, 1245-1248 (2001) [CrossRef] [PubMed]
  12. A. Wax, C. Yang, V. Backman, M. Kalashnikov, R. R. Dasari, and M. S. Feld, "Determination of particle size by using the angular distribution of backscattered light as measured with low-coherence interferometry," J. Opt. Soc. Am A 19, 737-744 (2002) [CrossRef]
  13. A. Wax, C. Yang, and J. A. Izatt, "Fourier-domain low-coherence interferometry for light-scattering spectroscopy," Opt. Lett. 28, 1230-1232 (2003) [CrossRef] [PubMed]
  14. J. Beuthan, O. Minet, J. Helfmann, M. Herrig, and G. Muller, "The spatial variation of the refractive index in biological cells," Phys. Med. Biol. 41, 369-382 (1996) [CrossRef] [PubMed]
  15. P. Sloot, A. Hoekstra, and C. Figdor, "Osmotic response of lymphocytes measured by means of forward light scattering: theoretical considerations," Cytometry 9, 636-641 (1988) [CrossRef] [PubMed]
  16. A.V. Oppenheim, R.W. Schafer, and J.R. Buck, �??The chirp transform algorithm,�?? in Discrete-Time Signal Processing, M. Horton, ed. (Prentice Hall, Upper Saddle River, New Jersey, 1999), pp. 656-661
  17. L. Thrane, M.H. Frosz, T.M. Jorgensen, A. Tycho, H.T. Yura, and P.E. Andersen, �??Extraction of scattering parameters and attenuation compensation in optical coherence tomography images of multilayered tissue structures,�?? Opt. Lett. 29, 1641-1643 (2004) [CrossRef] [PubMed]
  18. J. M. Schmitt, S. H. Xiang, and K. M. Yung, "Speckle in optical coherence tomography," J. Biomed. Opt. 4, 95- 105 (1999) [CrossRef] [PubMed]
  19. H. van de Hulst, Light Scattering by Small Particles (Dover Publications, New York, New York, 1981)

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited