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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 23 — Nov. 15, 2004
  • pp: 5754–5759

Monitoring live cell viability: Comparative study of fluorescence, oblique incidence reflection and phase contrast microscopy imaging techniques

Sylvie Landry, Peter L. McGhee, Robert J. Girardin, and Werden J. Keeler  »View Author Affiliations

Optics Express, Vol. 12, Issue 23, pp. 5754-5759 (2004)

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The relative performances of fluorescence, oblique incidence reflection and phase contrast imaging techniques have been studied for the purpose of monitoring long-term cellular activity and cell viability of several types of normal and cancerous cells in cultures. Time-lapse movies of live cell imaging of untagged and green fluorescent protein (GFP) tagged cell lines are presented. Oblique incidence reflection microscopy is the simplest and least expensive method to implement, appears to be the least phototoxic to cells, and is recommended for use in long-term optical monitoring of cell viability.

© 2004 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0180) Medical optics and biotechnology : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Research Papers

Original Manuscript: September 16, 2004
Revised Manuscript: November 5, 2004
Published: November 15, 2004

Sylvie Landry, Peter McGhee, Robert Girardin, and Werden Keeler, "Monitoring live cell viability: Comparative study of fluorescence, oblique incidence reflection and phase contrast microscopy imaging techniques," Opt. Express 12, 5754-5759 (2004)

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