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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 25 — Dec. 13, 2004
  • pp: 6178–6183

Rapid, depth-resolved light scattering measurements using Fourier domain, angle-resolved low coherence interferometry

John W. Pyhtila and Adam Wax  »View Author Affiliations

Optics Express, Vol. 12, Issue 25, pp. 6178-6183 (2004)

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We present a novel angle-resolved low coherence interferometry scheme for rapid measurement of depth-resolved angular scattering distributions to enable determination of scatterer size via elastic scattering properties. Depth resolution is achieved using a superluminescent diode in a modified Mach-Zehnder interferometer with the mixed signal and reference fields dispersed by an imaging spectrograph. The spectrograph slit is located in a Fourier transform plane of the scattering sample, enabling angle-resolved measurements over a 0.21 radian range. The capabilities of the new technique are demonstrated by recording the distribution of light scattered by a sub-surface layer of polystyrene microspheres in 40 milliseconds. The data are used to determine the microsphere size with good accuracy. Future clinical application to measuring the size of cell nuclei in living epithelial tissues using backscattered light is discussed.

© 2004 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(290.1350) Scattering : Backscattering

ToC Category:
Research Papers

Original Manuscript: October 21, 2004
Revised Manuscript: November 22, 2004
Published: December 13, 2004

John W. Pyhtila and Adam Wax, "Rapid, depth-resolved light scattering measurements using Fourier domain, angle-resolved low coherence interferometry," Opt. Express 12, 6178-6183 (2004)

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  1. A. Wax, C.H. Yang, R.R. Dasari, and M.S. Feld, "Measurement of angular distributions by use of low-coherence interferometry for light-scattering spectroscopy," Opt. Lett. 26, 322-324 (2001). [CrossRef]
  2. A. Wax, C. Yang, V. Backman, M. Kalashnikov, R.R. Dasari, and M.S. Feld, "Determination of particle size using the angular distribution of backscattered light as measured with low-coherence interferometry," J. Opt. Soc. Am. A 19, 737-744 (2002). [CrossRef]
  3. J.W. Pyhtila, R.N. Graf, and A. Wax, "Determining nuclear morphology using an improved angle-resolved low coherence interferometry system," Opt. Express 11, 3473-3484 (2003). <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-25-3473">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-25-3473</a> [CrossRef] [PubMed]
  4. A. Wax, C. Yang, V. Backman, K. Badizadegan, C.W. Boone, R.R. Dasari, and M.S. Feld, "Cell organization and sub-structure measured using angle-resolved low coherence interferometry.," Biophys. J. 82, 2256-2264 (2002). [CrossRef] [PubMed]
  5. A. Wax, C.H. Yang, M.G. Muller, R. Nines, C.W. Boone, V.E. Steele, G.D. Stoner, R.R. Dasari, and M.S. Feld, "In situ detection of neoplastic transformation and chemopreventive effects in rat esophagus epithelium using angle-resolved low-coherence interferometry," Cancer Res. 63, 3556-3559 (2003). [PubMed]
  6. A. Wax, J.W. Pyhtila, R.N. Graf, R. Nines, C.W. Boone, R.R. Dasari, M.S. Feld, V.E. Steele, and G.D. Stoner, "In situ monitoring of neoplastic transformation and assessing efficacy of chemopreventive agents in rat esophagus epithelium using angle-resolved low-coherence interferometry," Proceedings of the AACR, Volume 45, (2004).
  7. M.A. Choma, M.V. Sarunic, C.H. Yang, and J.A. Izatt, "Sensitivity advantage of swept source and Fourier domain optical coherence tomography," Opt. Express 11, 2183-2189 (2003). <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-18-2183">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-18-2183</a> [CrossRef] [PubMed]
  8. J.F. de Boer,, B. Cense, B.H. Park, M.C. Pierce, G.J. Tearney, and B.E. Bouma, "Improved signal-to-noise ratio in spectral-domain compared with time-domain optical coherence tomography," Opt. Lett. 28, 2067- 2069 (2003). [CrossRef] [PubMed]
  9. R. Leitgeb, C.K. Hitzenberger, and A.F. Fercher, "Performance of fourier domain vs. time domain optical coherence tomography," Opt. Express 11, 889-894 (2003). <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-8-889">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-8-889</a> [CrossRef] [PubMed]
  10. R.A. Leitgeb, W. Drexler, A. Unterhuber, B. Hermann, T. Bajraszewski, T. Le, A. Stingl, and A.F. Fercher, "Ultrahigh resolution Fourier domain optical coherence tomography," Opt. Express 12, 2156-2165 (2004). <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-10-2156">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-10-2156</a> [CrossRef] [PubMed]
  11. N.A. Nassif, B. Cense, B.H. Park, M.C. Pierce, S.H. Yun, B.E. Bouma, G.J. Tearney, T.C. Chen, and J.F. de Boer, "In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve," Opt. Express 12, 367-376 (2004). <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-3-367">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-3-367</a> [CrossRef] [PubMed]
  12. M. Wojtkowski, V.J. Srinivasan, T.H. Ko, J.G. Fujimoto, A. Kowalczyk, and J.S. Duker, "Ultrahighresolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation," Opt. Express 12, 2404-2422 (2004), <a href= "http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2404">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-12-11-2404</a> [CrossRef] [PubMed]
  13. Y.L. Kim, Y. Liu, R.K. Wali, H.K. Roy, M.J. Goldberg, A.K. Kromin, K. Chen, and V. Backman, "Simultaneous measurement of angular and spectral properties of light scattering for characterization of tissue microarchitecture and its alteration in early precancer," IEEE J. Sel. Top. Quant. Elec. 9, 243-256 (2003). [CrossRef]
  14. H.K. Roy, Y. Liu, R.K. Wali, Y.L. Kim, A.K. Kromine, M.J. Goldberg, and V. Backman, "Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis," Gastroenterology 126(4): p. 1071-1081 (2004). [CrossRef]

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