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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 25 — Dec. 13, 2004
  • pp: 6208–6214

Real-time Raman spectroscopy of optically trapped living cells and organelles

Changan Xie, Charles Goodman, Mumtaz A. Dinno, and Yong-Qing Li  »View Author Affiliations


Optics Express, Vol. 12, Issue 25, pp. 6208-6214 (2004)
http://dx.doi.org/10.1364/OPEX.12.006208


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Abstract

We report on real-time Raman spectroscopic studies of optically trapped living cells and organelles using an inverted confocal laser-tweezers-Raman-spectroscopy (LTRS) system. The LTRS system was used to hold a single living cell in a physiological solution or to hold a functional organelle within a living cell and consequently measured its Raman spectra. We have measured the changes in Raman spectra of a trapped yeast cell as the function of the temperature of the bathing solution and studied the irreversible cell degeneration during the heat denaturation. In addition, we measured the in-vitro Raman spectra of the nuclei within living pine cells and B. sporeformer, Strep. salivarius, and E. coli bacteria suspended in solution and showed the possibility of using LTRS system as a sensor for rapid identification of microbes in a fluid.

© 2004 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.5660) Medical optics and biotechnology : Raman spectroscopy

ToC Category:
Research Papers

History
Original Manuscript: October 18, 2004
Revised Manuscript: November 29, 2004
Published: December 13, 2004

Citation
Changan Xie, Charles Goodman, Mumtaz Dinno, and Yong-Qing Li, "Real-time Raman spectroscopy of optically trapped living cells and organelles," Opt. Express 12, 6208-6214 (2004)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-12-25-6208


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