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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 26 — Dec. 27, 2004
  • pp: 6440–6445

Manipulation and growth of birefringent protein crystals in optical tweezers

Wolfgang Singer, Halina Rubinsztein-Dunlop, and Ursula Gibson  »View Author Affiliations


Optics Express, Vol. 12, Issue 26, pp. 6440-6445 (2004)
http://dx.doi.org/10.1364/OPEX.12.006440


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Abstract

We report on the trapping, rotation, and in-situ growth of birefringent tetragonal lysozyme crystals in optical tweezers operating at a wavelength of 1070 nm. Variation of the pH and lysozyme concentration of the solution during growth was used to alter the length to width ratio of the crystals, and hence their orientation in the tweezers. Crystals with the optical axis skewed or perpendicular to the trapping-beam axis could be rotated by changing the orientation of linearly polarized light. We observed spontaneous spinning of some asymmetric crystals in the presence of linearly polarized light, due to radiation pressure effects. Addition of protein to the solution in the tweezers permitted real-time observation of crystal growth.

© 2004 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(260.5430) Physical optics : Polarization
(290.5850) Scattering : Scattering, particles

ToC Category:
Research Papers

History
Original Manuscript: November 12, 2004
Revised Manuscript: December 9, 2004
Published: December 27, 2004

Citation
Wolfgang Singer, Halina Rubinsztein-Dunlop, and Ursula Gibson, "Manipulation and growth of birefringent protein crystals in optical tweezers," Opt. Express 12, 6440-6445 (2004)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-12-26-6440


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References

  1. A. Ashkin, �??Applications of laser-radiation pressure,�?? Science 210, 1081-1088 (1980) [CrossRef] [PubMed]
  2. D. G. Grier, �??A revolution in optical manipulation,�?? Nature 424, 810-816 (2003) [CrossRef] [PubMed]
  3. J. E. Molloy and M. J. Padgett, �??Lights, action: optical tweezers,�?? Contemp. Phys. 43, 241-258 (2002) [CrossRef]
  4. R. C. Gauthier, �??Theoretical investigation of the optical trapping force and torque on cylindrical objects,�?? J. Opt. Soc. Am. B. 14, 3323-3333 (1997) [CrossRef]
  5. R. C. Gauthier, M. Ashman, and C. P. Grover, �??Experimental confirmation of the optical-trapping properties of cylindrical objects,�?? Appl. Opt. 38, 4861-4869 (1999) [CrossRef]
  6. A. T. O�??Neil and M. J. Padgett, �??Rotational control within optical tweezers by the use of a rotating aperture,�?? Opt. Lett. 27, 743-745 (2002) [CrossRef]
  7. S. J. Parkin , T. A. Nieminen , N. R.Heckenberg and H. Rubinsztein-Dunlop, �??Optical measurement of torque exerted on an elongated object by a noncircular laser beam,�?? Phys. Rev.A 70, Art. No. 023816 (2004) [CrossRef]
  8. P. Galajda and P. Ormos, �??Orientation of flat particles in optical tweezers by linearly polarized light�??, Optics Express 11, 446-451 (2003) [CrossRef] [PubMed]
  9. T. Wohland, A. Rosin, and E. H. K. Stelzer, �??Theoretical determination of the influence of the polarization on forces exerted by optical tweezers,�?? Optik 102, 181-190 (1996)
  10. E. Higurashi, R. Sawada, and T Ito, �??Optically driven angular alignment of microcomponents made of in-plane birefringent polyimide film based on optical angular momentum transfer,�?? J. Micromech. Microeng. 11, 140-145(2001) [CrossRef]
  11. M.E. Friese, T.A. Niemenen, N. R. Heckenberg, and H. Rubinsztein-Dunlop �??Optical alignment and spinning of laser-trapped microscopic particles,�?? Nature 394, 348-350 (1998) [CrossRef]
  12. E. Higurashi, R. Sawada, and T. Ito �??Optically induced angular alignment of trapped birefringent micro-objects by linearly polarized light,�?? Phys. Rev. E 59, 3676-3681 (1999) [CrossRef]
  13. A. LaPorta and M. Wang, �??Optical torque wrench: angular trapping, rotation and torque detection of quartz microparticles,�?? Phys. Rev. Lett. 92, Art. No. 190801 (2004) [CrossRef]
  14. E. Higurashi, H. Ukita, H. Tanaka, O. Ohguchi, �??Optically induced rotation of anisotropic micro-objects fabricated by surface micromachining,�?? Appl. Phys. Lett. 64, 2209-2210 (1994) [CrossRef]
  15. A. Yamamoto and I. Yamaguchi, �??Measurement and control of optically induced rotation of anisotropic shaped particles,�?? Jpn. J. Appl. Phys 34, 3104-3108 (1995) [CrossRef]
  16. J. Kobayashi, T. Asahi, M. Sakurai, I. Kagomiya, H. Asai, and H. Asami, �??The optical activity of lysozyme crystals,�?? Acta Cryst. A54, 581-590 (1998)
  17. K. Nakazato, T. Homma and T. Tomo �??Rapid solubility measurement of protein crystals as a function of precipitant concentration with micro-dialysis cell and two-beam interferometer,�?? J. Synchrotron Rad.. 11, 34-37 (2004) [CrossRef]
  18. W.J. Fredericks, M.C. Hammonds, S.B. Howard, F. Rosenberger �??Density, thermal expansivity, viscosity and refractive index of lysozyme solutions at crystal growth concentrations,�?? J. of Cryst. Growth 141, 183-192 (1994) [CrossRef]
  19. P. A. Bancel, V. B. Cajipe, and F. Rodier, �??Manipulating crystals with light,�?? J. Cryst. Growth 196, 685-690, (1999) [CrossRef]
  20. A. I. Bishop, T.A. Niemenen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, �??Optical applications and measurement of torque on microparticles of isotropic non-absorbing material,�?? Phys Rev. A 68, 033802 (2003) [CrossRef]
  21. N.E. Chayen , J. W. Radcliffe and D. M. Blow, �??Control Of Nucleation In The Crystallization Of Lysozyme,�?? Prot. Sci. 2, 113-118 ( 1993) [CrossRef]
  22. E.L.Forsythe, A. Nadarajah, and M. L. Pusey, �??Growth of (101) faces of tetragonal lysozyme crystals: measured growth-rate trends,�?? Acta Crystal. D 55, 1005-1011, (1999) [CrossRef]

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