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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 26 — Dec. 27, 2004
  • pp: 6440–6445

Manipulation and growth of birefringent protein crystals in optical tweezers

Wolfgang Singer, Halina Rubinsztein-Dunlop, and Ursula Gibson  »View Author Affiliations

Optics Express, Vol. 12, Issue 26, pp. 6440-6445 (2004)

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We report on the trapping, rotation, and in-situ growth of birefringent tetragonal lysozyme crystals in optical tweezers operating at a wavelength of 1070 nm. Variation of the pH and lysozyme concentration of the solution during growth was used to alter the length to width ratio of the crystals, and hence their orientation in the tweezers. Crystals with the optical axis skewed or perpendicular to the trapping-beam axis could be rotated by changing the orientation of linearly polarized light. We observed spontaneous spinning of some asymmetric crystals in the presence of linearly polarized light, due to radiation pressure effects. Addition of protein to the solution in the tweezers permitted real-time observation of crystal growth.

© 2004 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(260.5430) Physical optics : Polarization
(290.5850) Scattering : Scattering, particles

ToC Category:
Research Papers

Original Manuscript: November 12, 2004
Revised Manuscript: December 9, 2004
Published: December 27, 2004

Wolfgang Singer, Halina Rubinsztein-Dunlop, and Ursula Gibson, "Manipulation and growth of birefringent protein crystals in optical tweezers," Opt. Express 12, 6440-6445 (2004)

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