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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 3 — Feb. 9, 2004
  • pp: 449–457

Fast 3-D optical imaging with transient fluorescence signals

Haiyong Quan and Zhixiong Guo  »View Author Affiliations

Optics Express, Vol. 12, Issue 3, pp. 449-457 (2004)

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A fast 3-D optical imaging method with use of exogenous fluorescence agent is proposed and demonstrated by simulation in a model tissue. After administration of fluorescent agent, ultrashort near-infrared laser pulses are used to illuminate the tissue and excite fluorescence emission. The transient fluorescence signals are detected on the tissue boundaries and employed to reconstruct a 3-D image of relative fluorescence emission distribution inside the tissue. A region with greater fluorescence emission represents a diseased tissue if the fluorescent agent has a close affinity with the disease. We successfully demonstrated the feasibility of this method in the imaging of a small cubic tumor embedded in a cubical tissue phantom with a preassigned uptake distribution of fluorescent indocyanine green dye. The image reconstruction does not involve any inverse optimization. It took less than 5 minutes in a general PC for the two model imaging problems.

© 2004 Optical Society of America

OCIS Codes
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(260.2510) Physical optics : Fluorescence

ToC Category:
Research Papers

Original Manuscript: October 24, 2003
Revised Manuscript: January 26, 2004
Published: February 9, 2004

Haiyong Quan and Zhixiong Guo, "Fast 3-D Optical Imaging With Transient Fluorescence Signals," Opt. Express 12, 449-457 (2004)

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