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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 4 — Feb. 23, 2004
  • pp: 670–678

Optical trapping and fluorescence excitation with violet diode lasers and extended cavity surface emitting lasers

Tanya K. Lake, Antonia E. Carruthers, Lynn Paterson, Margaret Taylor, Frank Gunn-Moore, John W. Allen, Wilson Sibbett, and Kishan Dholakia  »View Author Affiliations


Optics Express, Vol. 12, Issue 4, pp. 670-678 (2004)
http://dx.doi.org/10.1364/OPEX.12.000670


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Abstract

Violet diode lasers and vertical extended cavity surface emitting lasers are used within an optical trapping system. Two distinct but related studies are performed. Firstly, the optical trapping efficiency in terms of the Q parameter for micron and sub-micron sized particles is determined. Secondly, we use the violet diode laser to observe and fluoresce 4′-6-Diamidino-2-phenylindole (DAPI) stained chromosomes, green fluorescent protein (GFP) transfected neuroblastomas and fluorescent polymer spheres within an optical tweezers using a 1064nm trap laser. This work paves the way for both reduced trap volumes and for biological tagging, chromosome selection or observing protein dynamics.

© 2004 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology

ToC Category:
Research Papers

History
Original Manuscript: November 10, 2003
Revised Manuscript: February 9, 2004
Published: February 23, 2004

Citation
Tanya Lake, Antonia Carruthers, Margaret Taylor, Lynn Paterson, Frank Gunn-Moore, John Allen, Wilson Sibbett, and Kishan Dholakia, "Optical trapping and fluorescence excitation with violet diode lasers and extended cavity surface emitting lasers," Opt. Express 12, 670-678 (2004)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-12-4-670


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References

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