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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 5 — Mar. 8, 2004
  • pp: 853–858

Detection of biological tissue in gels using pulsed digital holography

Maria del Socorro Hernández-Montes, C. Pérez-López, Fernando Mendoza Santoyo, and Luis Manuel Muñoz Guevara  »View Author Affiliations

Optics Express, Vol. 12, Issue 5, pp. 853-858 (2004)

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An out of plane optical sensitive configuration for pulsed digital holography was used to detect biological tissue inside solid organic materials like gels. A loud speaker and a shaker were employed to produce a mechanical wave that propagates through the gel in such a way that it generates vibrational resonant modes and transient events on the gel surface. Gel surface micro displacements were observed between the firing of two laser pulses, both for a steady resonant mode and for different times during the transient event. The biological tissue sample inserted approximately 2 cm inside the gel diffracts the original mechanical wave and changes the resonant mode pattern or the transient wave on the gel surface. This fact is used to quantitatively measure the gel surface micro displacement. Comparison of phase unwrapped patterns, with and without tissue inside the gel, allows the rapid identification of the existence of tissue inside the gel. The results for the resonant and transient conditions show that the method may be reliably used to study, compare and distinguish data from inside homogeneous and in-homogeneous solid organic materials.

© 2004 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Research Papers

Original Manuscript: January 16, 2004
Revised Manuscript: February 25, 2004
Published: March 8, 2004

Maria del Socorro Hernández-Montes, C. Pérez-López, Fernando Mendoza Santoyo, and Luis Manuel Guevara, "Detection of biological tissue in gels using pulsed digital holography," Opt. Express 12, 853-858 (2004)

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