OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 1 — Jan. 10, 2005
  • pp: 89–95

Serial noninvasive photoacoustic imaging of neovascularization in tumor angiogenesis

R.I. Siphanto, K.K. Thumma, R.G.M. Kolkman, T.G. van Leeuwen, F.F.M. de Mul, J.W. van Neck, L.N.A. van Adrichem, and W. Steenbergen  »View Author Affiliations

Optics Express, Vol. 13, Issue 1, pp. 89-95 (2005)

View Full Text Article

Enhanced HTML    Acrobat PDF (603 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present photoacoustic images of tumor neovascularization obtained over a 10-day period after subcutaneous inoculation of pancreatic tumor cells in a rat. The images were obtained from ultrasound generated by absorption in hemoglobin of short laser pulses at a wavelength of 1064 nm. The ultrasound signals were measured in reflection mode using a single scanning piezodetector, and images were reconstructed with a weighted delay-and-sum algorithm. Three-dimensional data visualize the development and quantify the extent of individual blood vessels around the growing tumor, blood concentration changes inside the tumor and growth in depth of the neovascularized region.

© 2005 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(300.1030) Spectroscopy : Absorption

ToC Category:
Research Papers

Original Manuscript: November 3, 2004
Revised Manuscript: December 17, 2004
Manuscript Accepted: December 21, 2004
Published: January 10, 2005

R.I. Siphanto, K.K. Thumma, R.G.M. Kolkman, T.G. van Leeuwen, F.F.M. de Mul, J.W. van Neck, L.N.A. van Adrichem, and W. Steenbergen, "Serial noninvasive photoacoustic imaging of neovascularization in tumor angiogenesis," Opt. Express 13, 89-95 (2005)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. J.  Folkman, “Angiogenesis in cancer, vascular, rheumatoid and other disease,” Nat. Med. 1, 27–31 (1995). [CrossRef] [PubMed]
  2. J.  Folkman, “New perspectives in clinical oncology from angiogenesis research,” Eur. J. Cancer 32A, 2534–2539 (1996). [CrossRef] [PubMed]
  3. J.  Hasan, R.  Byers, G.C.  Jayson, “Intra-tumoral microvessel density in human solid tumors,” Br. J. Cancer 86, 1566–1577 (2002). [CrossRef] [PubMed]
  4. M.W.  Dewhirst, B.  Klitzman, R.D.  Braun, D.M.  Brizel, Z.A.  Haroon, T.W.  Secomb, “Review of methods used to study oxygen transport at the microcirculatory level,” Int. J. Cancer 90, 237–255 (2000). [CrossRef] [PubMed]
  5. M.D.  Menger, M.W.  Laschke, B  Vollmar, “Viewing the microcirculation through the window: some twenty years experience with the hamster dorsal skinfold chamber,” Eur. Surg. Res. 34, 83–91 (2002). [CrossRef] [PubMed]
  6. M.V.  Knopp, H.  von Tengg-Kobligk, P.L.  Choyke, “Functional magnetic resonance imaging in oncology for diagnosis and therapy monitoring,” Mol. Cancer Ther. 2, 419–426 (2003). [PubMed]
  7. M.  Krix, F.  Kiessling, S.  Vosseler, N.  Farhan, M.M.  Mueller, P.  Bohlen, N.E.  Fusenig, S.  Delorme, “Sensitive noninvasive monitoring of tumor perfusion during antiangiogenic therapy by intermittent bolus-contrast power Doppler sonography,” Cancer Res. 63, 8264–8270 (2003). [PubMed]
  8. H.  Anderson, P.  Price, M.  Blomley, M.O.  Leach, P  Workman, “Measuring changes in human tumor vasculature in response to therapy using functional imaging techniques,” Br. J. Cancer 85, 1085–1093 (2001). [CrossRef] [PubMed]
  9. T.L.  Collier, R.  Lecomte, T.J.  McCarthy, S.  Meikle, T.J.  Ruth, F.  Scopinaro, A.  Signore, H.  VanBrocklin, C.  Van de Wiele, R.N.  Waterhouse, “Assessment of cancer-associated biomarkers by positron emission tomography: advances and challenges,” Dis. Markers 18, 211–247 (2002).
  10. C.G.A.  Hoelen, F.F.M.  de Mul, R.  Pongers, A.  Dekker, “Three-dimensional photoacoustic imaging of blood vessels in tissue,” Opt. Lett. 23, 648–650 (1998). [CrossRef]
  11. X.D.  Wang, Y.J.  Pang, G.  Ku G, X.Y.  Xie, G  Stoica, L.H.V.  Wang, “Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain,” Nat. Biotechnol. 21, 803–806 (2003). [CrossRef] [PubMed]
  12. A.A.  Karabutov, V.A.  Andreev, B.A.  Bell, R.D.  Fleming, Z.  Gatalica, M.  Motamedi, E.V.  Savateeva, H.  Singh, S.V.  Solomatin, S.L.  Thomsen, M.  Henrichs, A.A.  Oraevsky, “Optoacoustic images of early cancer in forward and backward modes,” in Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications , A.  Boccara, A.A.  Oraevsky; eds., Proc. SPIE 4434, 13–27 (2001).
  13. A.A.  Oraevsky, A.A.  Karabutov, S.V.  Solomatin, E.V.  Savateeva, V.A.  Andreev, Z.  Gatalica, H.  Singh, R.D.  Fleming, “Laser optoacoustic imaging of breast cancer in vivo,” in Biomedical Optoacoustics II, Proc. SPIE 4256, 6–15 (2001).
  14. R.A.  Kruger, K.D.  Miller, H.E.  Reynolds, W.L.  Kiser, D.R.  Reinecke, G.A.  Kruger, “Breast cancer in vivo: contrast enhancement with thermoacoustic CT at 434 MHz-feasibility study,” Radiology 216, 279–283 (2000). [PubMed]
  15. C.G.A.  Hoelen, F.F.M.  de Mul, “Image reconstruction for photoacoustic scanning of tissue structures,” Appl. Opt. 39, 5872–5883 (2000). [CrossRef]
  16. M.C.  Pilatou, N.J.  Voogd, F.F.M.  de Mul, W.  Steenbergen, L.N.A.  van Adrichem, “Analysis of three-dimensional photoacoustic imaging of a vascular tree in vitro,” Rev. Sci. Instrum. 74, 4495–4499 (2003). [CrossRef]
  17. C.G.H.  Hoelen, A.  Dekker, F.F.M.  de Mul, “Detection of photoacoustic transients originating from microstructures in optically diffuse media such as biological tissue”, IEEE Trans. Ultrason. Ferroelectr. Freq. Control 48, 37–47 (2001). [CrossRef] [PubMed]
  18. R.A.  Kruger, W.L.  Kiser, D.R.  Reinecke, G.A.  Kruger, “Thermoacoustic computed tomography using a conventional linear transducer array,” Med. Phys. 30, 856–860 (2003). [CrossRef] [PubMed]
  19. C.G.A.  Hoelen, F.F.M.  de Mul, “A new theoretical approach to photoacoustic signal generation,” J. Acoust. Soc. Am. 106, 695–706 (1999). [CrossRef]
  20. S.  Achilefu, R.B.  Dorshow, J.E.  Bugaj, R.  Rajagopalan, “Novel receptor-targeted fluorescent contrast agents for in vivo tumor imaging,”. Invest. Radiol. 35, 479–485 (2000). [CrossRef] [PubMed]
  21. P.  Carmeliet, R.K.  Jain, “Angiogenesis in cancer and other diseases,” Nature 407, 249–257, (2000). [CrossRef] [PubMed]
  22. S.  Ramanujan, G.C.  Koenig, T.P.  Padera, B.R.  Stoll, R.K.  Jain, “Local imbalance of proangiogenic and antiangiogenic factors: a potential mechanism of focal necrosis and dormancy in tumors,” Cancer Res. 60, 1442–1448 (2000). [PubMed]
  23. G.L.  Semenza, “Hypoxia-inducible factor 1: master regulator of O2 homeostasis,” Curr. Opin. Genet. Dev. 8, 588–594 (1998). [CrossRef] [PubMed]
  24. S.J.  van Belle, V.  Cocquyt, “Impact of haemoglobin levels on the outcome of cancers treated with chemotherapy,” Crit. Rev. Oncol. Hematol. 47, 1–11 (2003). [CrossRef] [PubMed]
  25. X.D.  Wang, G.  Ku, M.A.  Wegiel, D.J.  Bornhop, G.  Stoica, L.H.V.  Wang, “Noninvasive photoacoustic angiography of animal brains in vivo with near-infrared light and an optical contrast agent,” Opt. Lett. 29, 730–732 (2004). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited