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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 11 — May. 30, 2005
  • pp: 4263–4274

Modeling of spectral changes for depth localization of fluorescent inclusion

Jenny Svensson and Stefan Andersson-Engels  »View Author Affiliations


Optics Express, Vol. 13, Issue 11, pp. 4263-4274 (2005)
http://dx.doi.org/10.1364/OPEX.13.004263


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Abstract

We have performed modeling of fluorescence signals from inclusions inside turbid media to investigate the influence of a limited fluorescence contrast and how accurately the depth can be determined by using the spectral information. The depth was determined by forming a ratio of simulated fluorescence intensities at two wavelengths. The results show that it is important to consider the background autofluorescence in determining the depth of a fluorescent inclusion. It is also necessary to know the optical properties of the tissue to obtain the depth. A 20% error in absorption or scattering coefficients yields an error in the determined depth of approximately 2–3 mm (relative error of 10–15%) in a 20 mm thick tissue slab.

© 2005 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.5280) Medical optics and biotechnology : Photon migration
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Research Papers

History
Original Manuscript: April 27, 2005
Revised Manuscript: May 20, 2005
Published: May 30, 2005

Citation
Jenny Svensson and Stefan Andersson-Engels, "Modeling of spectral changes for depth localization of fluorescent inclusion," Opt. Express 13, 4263-4274 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-11-4263


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