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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 12 — Jun. 13, 2005
  • pp: 4693–4698

Nuclear morphology measurements using Fourier domain low coherence interferometry

Robert N. Graf and Adam Wax  »View Author Affiliations


Optics Express, Vol. 13, Issue 12, pp. 4693-4698 (2005)
http://dx.doi.org/10.1364/OPEX.13.004693


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Abstract

We present a new common path configuration Fourier domain low coherence interferometry (fLCI) optical system and demonstrate its capabilities by presenting results which determine the size of cell nuclei in a monolayer of T84 epithelial cells. The optical system uses a white light source in a modified Michelson interferometer and a spectrograph for detection of the mixed signal and reference fields. Depth resolution is obtained from the Fourier transform of the measured spectrum which provides the axial spatial cross-correlation between the signal and reference fields. The spectral dependence of scattering by the samples is determined by windowing the spectrum to measure the scattering amplitude as a function of wavenumber. We present evidence that fLCI accurately measures the longitudinal profile of cell nuclei rather than the transverse profile.

© 2005 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.1530) Medical optics and biotechnology : Cell analysis
(290.1350) Scattering : Backscattering

ToC Category:
Research Papers

History
Original Manuscript: May 6, 2005
Revised Manuscript: June 3, 2005
Published: June 13, 2005

Citation
Robert Graf and Adam Wax, "Nuclear morphology measurements using Fourier domain low coherence interferometry," Opt. Express 13, 4693-4698 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-12-4693


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References

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