OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 12 — Jun. 13, 2005
  • pp: 4693–4698

Nuclear morphology measurements using Fourier domain low coherence interferometry

Robert N. Graf and Adam Wax  »View Author Affiliations

Optics Express, Vol. 13, Issue 12, pp. 4693-4698 (2005)

View Full Text Article

Enhanced HTML    Acrobat PDF (1334 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present a new common path configuration Fourier domain low coherence interferometry (fLCI) optical system and demonstrate its capabilities by presenting results which determine the size of cell nuclei in a monolayer of T84 epithelial cells. The optical system uses a white light source in a modified Michelson interferometer and a spectrograph for detection of the mixed signal and reference fields. Depth resolution is obtained from the Fourier transform of the measured spectrum which provides the axial spatial cross-correlation between the signal and reference fields. The spectral dependence of scattering by the samples is determined by windowing the spectrum to measure the scattering amplitude as a function of wavenumber. We present evidence that fLCI accurately measures the longitudinal profile of cell nuclei rather than the transverse profile.

© 2005 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.1530) Medical optics and biotechnology : Cell analysis
(290.1350) Scattering : Backscattering

ToC Category:
Research Papers

Original Manuscript: May 6, 2005
Revised Manuscript: June 3, 2005
Published: June 13, 2005

Robert Graf and Adam Wax, "Nuclear morphology measurements using Fourier domain low coherence interferometry," Opt. Express 13, 4693-4698 (2005)

Sort:  Journal  |  Reset  


  1. V. Backman, V. Gopal, M. Kalashnikov, K. Badizadegan, R. Gurjar, A. Wax, I. Georgakoudi, M. Mueller, C.W. Boone, R.R. Dasari, and M.S. Feld, "Measuring cellular structure at submicrometer scale with light scattering spectroscopy," IEEE. J. Sel. Top. Quantum Electron. 7, 887-893 (2001). [CrossRef]
  2. A. Wax, C.H. Yang, V. Backman, K. Badizadegan, C.W. Boone, R.R. Dasari, and M.S. Feld, "Cell organization and sub-structure measured using angle-resolved low coherence interferometry," Biophys. J. 82, 2256-2264 (2002). [CrossRef] [PubMed]
  3. V. Backman, M.B. Wallace, L.T. Perelman, J.T. Arendt, R. Gurjar, M.G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J.M. Crawford, M. Fitzmaurice, S. Kabani, H.S. Levin, M. Seiler, R.R. Dasari, I. Itzkan, J. Van Dam, and M.S. Feld, "Detection of preinvasive cancer cells," Nature 406, 35-36 (2000). [CrossRef] [PubMed]
  4. A. Wax, C.H. Yang, M.G. Muller, R. Nines, C.W. Boone, V.E. Steele, G.D. Stoner, R.R. Dasari, and M.S. Feld, "In situ detection of neoplastic transformation and chemopreventive effects in rat esophagus epithelium using angle-resolved low-coherence interferometry," Cancer Research 63, 3556-3559 (2003). [PubMed]
  5. A. Wax, J.W. Pyhtila, R.N. Graf, R. Nines, C.W. Boone, R.R. Dasari, M.S. Feld, V.E. Steele, and G.D. Stoner, " Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low coherence interferometry" J. Biomed. Opt. in press
  6. A. Wax, C.H. Yang, R.R. Dasari, and M.S. Feld, "Measurement of angular distributions by use of low-coherence interferometry for light-scattering spectroscopy," Optics Letters 26, 322-324 (2001). [CrossRef]
  7. A. Wax, C.H. Yang, V. Backman, M. Kalashnikov, R.R. Dasari, and M.S. Feld, "Determination of particle size using the angular distribution of backscattered light as measured with low-coherence interferometry," J. Opt. Soc. Am. A 19, 737-744 (2002). [CrossRef]
  8. A. Wax, C.H. Yang, and J.A. Izatt, "Fourier-domain low-coherence interferometry for light-scattering spectroscopy," Opt. Lett. 28, 1230-1232 (2003). [CrossRef] [PubMed]
  9. M. Wojtkowski, A. Kowalczyk, R. Leitgeb, and A.F. Fercher, "Full range complex spectral optical coherence tomography technique in eye imaging," Opt. Lett. 27, 1415-1417 (2002). [CrossRef]
  10. M. Wojtkowski, R. Leitgeb, A. Kowalczyk, T. Bajraszewski, and A.F. Fercher, "In vivo human retinal imaging by Fourier domain optical coherence tomography," J. Biomed. Opt. 7, 457-463 (2002). [CrossRef] [PubMed]
  11. R. Leitgeb, M. Wojtkowski, A. Kowalczyk, C.K. Hitzenberger, M. Sticker, and A.F. Fercher, "Spectral measurement of absorption by spectroscopic frequency- domain optical coherence tomography," Opt. Lett. 25, 820-822 (2000). [CrossRef]
  12. U. Morgner, W. Drexler, F.X. Krtner, X.D. Li, C. Pitris, E.P. Ippen, J.G. Fujimoto, �??Spectroscopic optical coherence tomography,�?? Opt. Lett. 25, 111-113 (2000). [CrossRef]
  13. V. Tuchin, �??Tissue optics: light scattering methods and instruments for medical diagnosis,�?? SPIE (2000).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited