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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 13 — Jun. 27, 2005
  • pp: 4828–4842

Determination of reduced scattering coefficient of biological tissue from a needle-like probe

Maureen Johns, Cole A. Giller, Dwight C. German, and Hanli Liu  »View Author Affiliations

Optics Express, Vol. 13, Issue 13, pp. 4828-4842 (2005)

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Detection of interactions between light and tissue can be used to characterize the optical properties of the tissue. The purpose of this paper is to develop an algorithm that determines the reduced scattering coefficient (µs) of tissues from a single optical reflectance spectrum measured with a small source-detector separation. A qualitative relationship between µs and optical reflectance was developed using both Monte Carlo simulations and empirical tissue calibrations for each of two fiber optic probes with 400-µm and 100-µm fibers. Optical reflectance measurements, using a standard frequency-domain oximeter, were performed to validate the calculated µs values. The algorithm was useful for determining µs values of in vivo human fingers and rat brain tissues.

© 2005 Optical Society of America

OCIS Codes
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(290.4210) Scattering : Multiple scattering

ToC Category:
Research Papers

Original Manuscript: April 22, 2005
Revised Manuscript: June 7, 2005
Published: June 27, 2005

Maureen Johns, Cole Giller, Dwight German, and Hanli Liu, "Determination of reduced scattering coefficient of biological tissue from a needle-like probe," Opt. Express 13, 4828-4842 (2005)

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