Study of dynamic process of acetic acid induced-whitening in epithelial tissues at cellular level

doi:10.1364/OPEX.13.004963

Optics Express

Editor: Michael Duncan
Vol. 13, Iss. 13 — Jun. 27, 2005
pp: 4963–4973

Study of dynamic process of acetic acid induced-whitening in epithelial tissues at cellular level

Tao T. Wu, Jianan Qu, Tak Hong Cheung, So Fan Yim, and Yick Fu Wong »View Author Affiliations

Optics Express, Vol. 13, Issue 13, pp. 4963-4973 (2005)
http://dx.doi.org/10.1364/OPEX.13.004963

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Abstract
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Abstract

Acetic acid, inducing transient whitening (acetowhitening) when applied to epithelial tissues, is a commonly used contrast agent for detecting early cervical cancer. The goals of this research are to investigate the temporal characteristics of acetowhitening process in cervical epithelial tissue at cellular level and develop a clear understanding of the diagnostic information carried in the acetowhitening signal. A system measuring time-resolved reflectance was built to study the rising and decay processes of acetowhitening signal from the monolayered cell cultures of normal and cancerous cervical squamous cells. It is found that the dynamic processes of acetowhitening in normal and cancerous cells are significantly different. The results of this study provide insight valuable to further understand the acetowhitening process in epithelial cells and to encourage the development of an objective procedure to detect the early cervical cancers based on quantitative monitoring of the dynamic process of acetowhitening.

OCIS Codes
(120.5820) Instrumentation, measurement, and metrology : Scattering measurements
(160.4760) Materials : Optical properties
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(290.1350) Scattering : Backscattering

ToC Category:
Research Papers

History
Original Manuscript: May 2, 2005
Revised Manuscript: June 14, 2005
Published: June 27, 2005

Citation
Tao T. Wu, Jianan Qu, Tak Hong Cheung, So Fan Yim, and Yick Fu Wong, "Study of dynamic process of acetic acid induced-whitening in epithelial tissues at cellular level," Opt. Express 13, 4963-4973 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-13-4963

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References

T. C. Wright, R. J. Kurman, and A. Ferenczy, �??Cervical Intraepithelial Neoplasia,�?? in Blaustein A. Pathology of the Female Genital Tract (Springer-Verlag, New York, 1994).
U.S. Preventive Services Task Force, Guide to clinical preventive services: report of the U.S. Preventive Services Task Force (Williams & Wilkins, 1996).
M. C. Anderson, J. A. Jordan, A. R. Morse, and F. Sharp, A Text and Atlas of Integrated Colposcopy: for Colposcopists, Histopathologists and Cytologists (Chapman & Hall Medical, 1992).
M. F. Mitchell, �??The Accuracy of Colposcopy,�?? Clinical Consultations in Obstetrics and Gynecology 6, 70-73, (1994).
M. Ronne, �??Chromosome preparation and high resolution banding techniques. A review,�?? J. Dairy Sci. 72, 1363-1377, (1989). [CrossRef] [PubMed]
S. Yamada, D. Wirtz, and P. A. Coulombe, �??Pairwise Assembly Determines the Intrinsic Potential for Self-Organization and Mechanical Properties of Keratin Filaments,�?? Mol. Biol. Cell 13, 382-391, (2002). [CrossRef] [PubMed]
R. Lambert, J. F. Rey, R. Sankaranarayanan, �??Magnification and Chromoscopy with the Acetic Acid Test,�?? Endoscopy 35, 437-445, (2003). [CrossRef] [PubMed]
C. Smithpeter, A. Dunn, R. Drezek, T. Collier, and R. Richards-Kortum, �??Near Real Time Confocal Microscopy of Cultured Amelanotic Cells: Sources of Signal, Contrast Agents, and Limits of Contrast,�?? J. Biomed. Opt. 3, 429�??436 (1998). [CrossRef]
B. W. Pogue, H. B. Kaufman, A. Zelenchuk, W. Harper, G. C. Burke, E. E. Burke, D. M. Harper, �??Analysis of Acetic Acid-Induced Whitening of High-Grade Squamous Intraepithelial Lesions,�?? J. Biomed. Opt. 6, 397-403, (2001). [CrossRef] [PubMed]
C. Balas, �??A Novel Optical Imaging Method for the Early Detection, Quantitative Grading, and Mapping of Cancerous and Precancerous Lesions of Cervix,�?? IEEE Trans. Biomed. Eng. 48, 96-104, (2001). [CrossRef] [PubMed]
R. S. Cotran, V. Kumar, and T. Collins, Robbins Pathologic Basis of Disease (W. B. Saunders, 1999).
M. A. Stanley, �??Culture of Human Cervical Epithelial Cells�??, in Culture of Epithelial Cells, R. I. Freshney and M. G. Freshney, eds. (Wiley-Liss, 2002).

Blaustein A.Pathology of the Female Gen

T. C. Wright, R. J. Kurman, and A. Ferenczy, �??Cervical Intraepithelial Neoplasia,�?? in Blaustein A. Pathology of the Female Genital Tract (Springer-Verlag, New York, 1994).

Clinical Consultations in Obstetrics and

M. F. Mitchell, �??The Accuracy of Colposcopy,�?? Clinical Consultations in Obstetrics and Gynecology 6, 70-73, (1994).

Culture of Epithelial Cells

M. A. Stanley, �??Culture of Human Cervical Epithelial Cells�??, in Culture of Epithelial Cells, R. I. Freshney and M. G. Freshney, eds. (Wiley-Liss, 2002).

Endoscopy

R. Lambert, J. F. Rey, R. Sankaranarayanan, �??Magnification and Chromoscopy with the Acetic Acid Test,�?? Endoscopy 35, 437-445, (2003). [CrossRef] [PubMed]

IEEE Trans. Biomed. Eng.

C. Balas, �??A Novel Optical Imaging Method for the Early Detection, Quantitative Grading, and Mapping of Cancerous and Precancerous Lesions of Cervix,�?? IEEE Trans. Biomed. Eng. 48, 96-104, (2001). [CrossRef] [PubMed]

J. Biomed. Opt.

C. Smithpeter, A. Dunn, R. Drezek, T. Collier, and R. Richards-Kortum, �??Near Real Time Confocal Microscopy of Cultured Amelanotic Cells: Sources of Signal, Contrast Agents, and Limits of Contrast,�?? J. Biomed. Opt. 3, 429�??436 (1998). [CrossRef]
B. W. Pogue, H. B. Kaufman, A. Zelenchuk, W. Harper, G. C. Burke, E. E. Burke, D. M. Harper, �??Analysis of Acetic Acid-Induced Whitening of High-Grade Squamous Intraepithelial Lesions,�?? J. Biomed. Opt. 6, 397-403, (2001). [CrossRef] [PubMed]

J. Dairy Sci.

M. Ronne, �??Chromosome preparation and high resolution banding techniques. A review,�?? J. Dairy Sci. 72, 1363-1377, (1989). [CrossRef] [PubMed]

Mol. Biol. Cell

S. Yamada, D. Wirtz, and P. A. Coulombe, �??Pairwise Assembly Determines the Intrinsic Potential for Self-Organization and Mechanical Properties of Keratin Filaments,�?? Mol. Biol. Cell 13, 382-391, (2002). [CrossRef] [PubMed]

Other

U.S. Preventive Services Task Force, Guide to clinical preventive services: report of the U.S. Preventive Services Task Force (Williams & Wilkins, 1996).
M. C. Anderson, J. A. Jordan, A. R. Morse, and F. Sharp, A Text and Atlas of Integrated Colposcopy: for Colposcopists, Histopathologists and Cytologists (Chapman & Hall Medical, 1992).
R. S. Cotran, V. Kumar, and T. Collins, Robbins Pathologic Basis of Disease (W. B. Saunders, 1999).

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