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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 13 — Jun. 27, 2005
  • pp: 5151–5156

Simple high-speed confocal line-scanning microscope

Kang-Bin Im, Sumin Han, Hwajoon Park, Dongsun Kim, and Beop-Min Kim  »View Author Affiliations

Optics Express, Vol. 13, Issue 13, pp. 5151-5156 (2005)

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Using a line scan camera and an acousto-optic deflector (AOD), we constructed a high-speed confocal laser line-scanning microscope that can generate confocal images (512×512 pixels) with up to 191 frames/s without any mechanically moving parts. The line scanner consists of an AOD and a cylindrical lens, which creates a line focus sweeping over the sample. The measured resolutions in z (depth), x (perpendicular to line focus), and y (direction of line focus) directions are 3.3 µm, 0.7 µm and 0.9 µm, respectively, with a 50× objective lens. This confocal microscope may be useful for analyzing fast phenomena during biological and chemical interactions and for fast 3D image reconstruction.

© 2005 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Research Papers

Original Manuscript: April 12, 2005
Revised Manuscript: May 12, 2005
Published: June 27, 2005

Kang-Bin Im, Sumin Han, Hwajoon Park, Dongsun Kim, and Beop-Min Kim, "Simple high-speed confocal line-scanning microscope," Opt. Express 13, 5151-5156 (2005)

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  1. M. Minsky, �??Microscopy apparatus,�?? U.S. patent 3,013,467 (December 1961).
  2. R. Y. Tsien and B. J. Bacskai, �??Video-rate confocal microscopy,�?? in Handbook of Biological Confocal Microscopy, J. B. Pawley (Plenum, New York, 1995), pp. 459�??478.
  3. C. J. Koester, �??Scanning mirror microscope with optical sectioning characteristics: applications in ophthalmology,�?? Appl. Opt. 19, 1749�??1757 (1980). [CrossRef] [PubMed]
  4. B. R. Masters and A. A. Thaer, �??Real-time scanning slit confocal microscopy of the in-vivo human cornea,�?? Appl. Opt. 33, 695�??701 (1994). [CrossRef] [PubMed]
  5. G. Q. Xiao, T. R. Corle, and G. S. Kino, �??Real-time confocal scanning optical microscope,�?? Appl. Phys. Lett. 53, 716�??718 (1988). [CrossRef]
  6. M. Rahadhyaksha, R. R. Anderson, and R. H. Webb, �??Video-rate confocal scanning laser microscope for imaging human tissues in vivo,�?? Appl. Opt. 38, 2105�??2115 (1999). [CrossRef]
  7. J. Cushion, F. N. Reinholz, and B. A. Patterson, �??General purpose control system for scanning laser ophthalmoscopes,�?? Clin. Exp. Ophthalmol. 31, 241�??245 (2003). [CrossRef]
  8. W. B. Amos and J. G. White, �??Direct view confocal imaging systems using a slit aperture,�?? in Handbook of Biological Confocal Microscopy, J. B. Pawley (Plenum, New York, 1995), pp. 403�??415.
  9. Y. S. Sabharwal, A. R. Rouse, L. Donaldson, M. F. Hopkins, and A. F. Gmitro, �??Slit-scanning confocal microendoscope for high-resolution in-vivo imaging,�?? Appl. Opt. 38, 7133�??7144 (1999). [CrossRef]
  10. A. F. Gmitro and D. Aziz, �??Confocal microscopy through a fiber-optic imaging bundle,�?? Opt. Lett. 18, 565�??567 (1993). [CrossRef] [PubMed]
  11. R. H. Webb, �??Confocal optical microscopy,�?? Rep. Prog. Phys. 59, 427�??471 (1996). [CrossRef]
  12. C. J. R. Sheppard and X. Q. Mao, �??Confocal microscopes with slit apertures,�?? J. Mod. Opt. 25, 1169 (1998).
  13. T. R. Corle, C.�??H. Chou, and G. S. Kino, �??Depth response of confocal optical microscope,�?? Opt. Lett. 11, 770�??772 (1986). [CrossRef] [PubMed]

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