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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 13 — Jun. 27, 2005
  • pp: 5151–5156

Simple high-speed confocal line-scanning microscope

Kang-Bin Im, Sumin Han, Hwajoon Park, Dongsun Kim, and Beop-Min Kim  »View Author Affiliations


Optics Express, Vol. 13, Issue 13, pp. 5151-5156 (2005)
http://dx.doi.org/10.1364/OPEX.13.005151


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Abstract

Using a line scan camera and an acousto-optic deflector (AOD), we constructed a high-speed confocal laser line-scanning microscope that can generate confocal images (512×512 pixels) with up to 191 frames/s without any mechanically moving parts. The line scanner consists of an AOD and a cylindrical lens, which creates a line focus sweeping over the sample. The measured resolutions in z (depth), x (perpendicular to line focus), and y (direction of line focus) directions are 3.3 µm, 0.7 µm and 0.9 µm, respectively, with a 50× objective lens. This confocal microscope may be useful for analyzing fast phenomena during biological and chemical interactions and for fast 3D image reconstruction.

© 2005 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Research Papers

History
Original Manuscript: April 12, 2005
Revised Manuscript: May 12, 2005
Published: June 27, 2005

Citation
Kang-Bin Im, Sumin Han, Hwajoon Park, Dongsun Kim, and Beop-Min Kim, "Simple high-speed confocal line-scanning microscope," Opt. Express 13, 5151-5156 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-13-5151


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