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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 14 — Jul. 11, 2005
  • pp: 5363–5368

Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source

J. A. Palero, V. O. Boer, J. C. Vijverberg, H. C. Gerritsen, and H. J. C. M. Sterenborg  »View Author Affiliations


Optics Express, Vol. 13, Issue 14, pp. 5363-5368 (2005)
http://dx.doi.org/10.1364/OPEX.13.005363


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Abstract

We report on a novel and simple light source for short-wavelength two-photon excitation fluorescence microscopy based on the visible nonsolitonic radiation from a photonic crystal fiber. We demonstrate tunability of the light source by varying the wavelength and intensity of the Ti:Sapphire excitation light source. The visible nonsolitonic radiation is used as an excitation light source for two-photon fluorescence microscopy of tryptophan powder.

© 2005 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(190.4180) Nonlinear optics : Multiphoton processes
(190.4370) Nonlinear optics : Nonlinear optics, fibers
(230.3990) Optical devices : Micro-optical devices

ToC Category:
Research Papers

History
Original Manuscript: June 6, 2005
Revised Manuscript: June 24, 2005
Published: July 11, 2005

Citation
J. Palero, V. Boer, J. Vijverberg, H. Gerritsen, and H. J. C. M. Sterenborg, "Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source," Opt. Express 13, 5363-5368 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-14-5363


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References

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