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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 15 — Jul. 25, 2005
  • pp: 5883–5889

Selective detection of antibodies in microstructured polymer optical fibers

Jesper B. Jensen, Poul E. Hoiby, Grigoriy Emiliyanov, Ole Bang, Lars H. Pedersen, and Anders Bjarklev  »View Author Affiliations

Optics Express, Vol. 13, Issue 15, pp. 5883-5889 (2005)

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We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fiber (mPOF). The fiber core is defined by a ring of 6 air holes and a simple procedure was applied to selectively capture either α-streptavidin or α-CRP antibodies inside these air holes. A sensitive and easy-to-use fluorescence method was used for the optical detection. Our results show that mPOF based biosensors can provide reliable and selective antibody detection in ultra small sample volumes.

© 2005 Optical Society of America

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(120.1880) Instrumentation, measurement, and metrology : Detection
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(230.3990) Optical devices : Micro-optical devices

ToC Category:
Research Papers

Original Manuscript: June 8, 2005
Revised Manuscript: July 18, 2005
Published: July 25, 2005

Jesper Jensen, Poul Hoiby, Grigoriy Emiliyanov, Ole Bang, Lars Pedersen, and Anders Bjarklev, "Selective detection of antibodies in microstructured polymer optical fibers," Opt. Express 13, 5883-5889 (2005)

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